Journal of Biological Control

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Effect of Different Grains as Solid Substrates on Sporulation, Viability and Pathogenicity of Metarhizium anisopliae (Metschnikoff) Sorokin


Affiliations
1 College of Forestry & Hill Agriculture, G B. Pant Univ. of Ag. & Tech. Hill Campus, Ranichauri, Tehri Garhwal - 249199 (Uttarakhand), India
2 Department of Entomology, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India
 

Influence of different cereal grains as solid substrates on sporulation, viability and pathogenicity of Metarhizium anisopliae (Metschnikoff) Sorokin was studied under laboratory condition. Among the six grains (viz. maize, sorghum, fingermillet, sorghum, barley and wheat), sorghum as solid substrate resulted in highest spore (6.36 × 107 conidia ml−1) production with spore viability of 86.6 per cent. The production and viability of conidia increased when the substrates were supplemented with sucrose as compared to conidia produced from substrates alone. Conidia produced from fingermillet showed highest virulence against third instar larvae of Spodoptera litura with LC50, value of 1,72 × 106 conidia ml−1 compared to conidia from other sources. However, conidia produced from SDA medium showed highest spore production, viability and virulence to S. litura larvae compared to conidia produced from solid substrates.

Keywords

Metarhizium, Pathogenicity, Solid Substrates Fermentation, Spodoptera Sporulation, Viability.
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  • Effect of Different Grains as Solid Substrates on Sporulation, Viability and Pathogenicity of Metarhizium anisopliae (Metschnikoff) Sorokin

Abstract Views: 204  |  PDF Views: 121

Authors

A. K. Pandey
College of Forestry & Hill Agriculture, G B. Pant Univ. of Ag. & Tech. Hill Campus, Ranichauri, Tehri Garhwal - 249199 (Uttarakhand), India
K. R. Kanaujia
Department of Entomology, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India

Abstract


Influence of different cereal grains as solid substrates on sporulation, viability and pathogenicity of Metarhizium anisopliae (Metschnikoff) Sorokin was studied under laboratory condition. Among the six grains (viz. maize, sorghum, fingermillet, sorghum, barley and wheat), sorghum as solid substrate resulted in highest spore (6.36 × 107 conidia ml−1) production with spore viability of 86.6 per cent. The production and viability of conidia increased when the substrates were supplemented with sucrose as compared to conidia produced from substrates alone. Conidia produced from fingermillet showed highest virulence against third instar larvae of Spodoptera litura with LC50, value of 1,72 × 106 conidia ml−1 compared to conidia from other sources. However, conidia produced from SDA medium showed highest spore production, viability and virulence to S. litura larvae compared to conidia produced from solid substrates.

Keywords


Metarhizium, Pathogenicity, Solid Substrates Fermentation, Spodoptera Sporulation, Viability.