Journal of Biological Control

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Molecular Characterisation of some Indian Anthocorid Predators


Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024, India
 

Anthocorid predators widely used as biocontrol agents were characterised for genetic diversity and phylogeny. Cardiastethus exiguus Poppius, Xylocoris flavipes Reuter and Blaptostethus pallescens Poppius were characterised using Cytochrome oxidase I (COI) marker to elucidate the diversity and phylogeny. The sequencing of the COI region at 650bp was compared with sequence of Orius majusculus (Reuter), available at NCBI by BLASTn. The cluster analysis based on dendogram revealed B. pallescens and O. majusculus to be more closely related, while X. flavipes and C. exiguus were distinct. The usefulness of characterisation and phylogenetic relationship among the anthocorid populations for their effective utilisation in pest management is discussed.

Keywords

Anthocorids, COI, PCR, Phylogeny, Predators.
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  • Molecular Characterisation of some Indian Anthocorid Predators

Abstract Views: 227  |  PDF Views: 135

Authors

T. L. Dheemanth
ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024, India
K. Srinivasa Murthy
ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024, India
Chandish R. Ballal
ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024, India

Abstract


Anthocorid predators widely used as biocontrol agents were characterised for genetic diversity and phylogeny. Cardiastethus exiguus Poppius, Xylocoris flavipes Reuter and Blaptostethus pallescens Poppius were characterised using Cytochrome oxidase I (COI) marker to elucidate the diversity and phylogeny. The sequencing of the COI region at 650bp was compared with sequence of Orius majusculus (Reuter), available at NCBI by BLASTn. The cluster analysis based on dendogram revealed B. pallescens and O. majusculus to be more closely related, while X. flavipes and C. exiguus were distinct. The usefulness of characterisation and phylogenetic relationship among the anthocorid populations for their effective utilisation in pest management is discussed.

Keywords


Anthocorids, COI, PCR, Phylogeny, Predators.