Journal of Biological Control

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Effect of Different Media on Growth and Sporulation of Metarhizium anisopliae, a Fungal Bioagent


Affiliations
1 N. D. University of Agriculture and Technology, Department of Plant Pathology, Crop Research Station, Bahraich 271801, Uttar Pradesh, India
 

An Influence of different culture media containing host extract as substrate on hyphal growth and sporulation of Metarhizium was evaluated in laboratory studied. The minimum average of 5.6 days was required for the sporulation of the fungus in Sabouraud's dextrose agar (SDA) medium supplemented with host extract (Pyrilla nymph/adult), where as in Emerson YPSS medium it was 7.3 days. The SDA medium in combination of host extract of the insect was found significantly superior over the other entire medium tested. Radial growth of 4.03 cm at days after inoculation (DAI) was observed in SDA medium supplemented with insect extract followed by SDA alone and Emerson YPSS medium with radial growth of 3.5 and 3.01 cm, respectively.

Keywords

Culture Media, Host Extract, Hyphal Growth, Metarhizium anisopliae, Sporulation.
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  • Effect of Different Media on Growth and Sporulation of Metarhizium anisopliae, a Fungal Bioagent

Abstract Views: 226  |  PDF Views: 113

Authors

Sunita Lal
N. D. University of Agriculture and Technology, Department of Plant Pathology, Crop Research Station, Bahraich 271801, Uttar Pradesh, India
D. C. Rajak
N. D. University of Agriculture and Technology, Department of Plant Pathology, Crop Research Station, Bahraich 271801, Uttar Pradesh, India
O. K. Sinha
N. D. University of Agriculture and Technology, Department of Plant Pathology, Crop Research Station, Bahraich 271801, Uttar Pradesh, India
V. Singh
N. D. University of Agriculture and Technology, Department of Plant Pathology, Crop Research Station, Bahraich 271801, Uttar Pradesh, India

Abstract


An Influence of different culture media containing host extract as substrate on hyphal growth and sporulation of Metarhizium was evaluated in laboratory studied. The minimum average of 5.6 days was required for the sporulation of the fungus in Sabouraud's dextrose agar (SDA) medium supplemented with host extract (Pyrilla nymph/adult), where as in Emerson YPSS medium it was 7.3 days. The SDA medium in combination of host extract of the insect was found significantly superior over the other entire medium tested. Radial growth of 4.03 cm at days after inoculation (DAI) was observed in SDA medium supplemented with insect extract followed by SDA alone and Emerson YPSS medium with radial growth of 3.5 and 3.01 cm, respectively.

Keywords


Culture Media, Host Extract, Hyphal Growth, Metarhizium anisopliae, Sporulation.