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Isolation and in vitro studies on indigenous fungi against ischolar_main-knot nematode, Meloidogyne incognita


Affiliations
1 Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu, India
2 Department of Plant Pathology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu, India
 

The present study was carried out to evaluate the indigenous fungi from soil against the ischolar_main knot nematode, Meloidogyne incognita. Eleven isolates were isolated and screened for their efficacy against nematode under in vitro condition for egg hatching and juvenile mortality. The results revealed that the isolate F6 showed the highest egg hatching inhibition (88.5%) followed by F11 and F7 compared to control after incubation for 72 h. The highest juvenile mortality of 73% was recorded in isolate F6 followed by F11 and F9 after 72 h. Isolate F6 was morphologically and molecularly identified as Talaromyces pinophilus and F9 and F11 were identified as Aspergillus corrugates and Aspergillus nidulans. Among the fungi, T. pinophilus was found to be highly effective against ischolar_main knot nematode.


Keywords

Indigenous fungi, culture filtrate, in vitro, Meloidogyne incognita, Talaromyces pinophilus
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  • Isolation and in vitro studies on indigenous fungi against ischolar_main-knot nematode, Meloidogyne incognita

Abstract Views: 203  |  PDF Views: 102

Authors

Nithya Dharshini K.
Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu, India
Jothi G.
Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu, India
Swarnakumari N.
Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu, India
L. Rajendran
Department of Plant Pathology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu, India

Abstract


The present study was carried out to evaluate the indigenous fungi from soil against the ischolar_main knot nematode, Meloidogyne incognita. Eleven isolates were isolated and screened for their efficacy against nematode under in vitro condition for egg hatching and juvenile mortality. The results revealed that the isolate F6 showed the highest egg hatching inhibition (88.5%) followed by F11 and F7 compared to control after incubation for 72 h. The highest juvenile mortality of 73% was recorded in isolate F6 followed by F11 and F9 after 72 h. Isolate F6 was morphologically and molecularly identified as Talaromyces pinophilus and F9 and F11 were identified as Aspergillus corrugates and Aspergillus nidulans. Among the fungi, T. pinophilus was found to be highly effective against ischolar_main knot nematode.


Keywords


Indigenous fungi, culture filtrate, in vitro, Meloidogyne incognita, Talaromyces pinophilus



DOI: https://doi.org/10.18311/jbc%2F2021%2F28554