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Biocontrol of Rhizoctonia solani Root Rot of Chilli by Bacillus subtilis formulations under Pot Conditions


Affiliations
1 Department of Microbiology, Center for PG Studies, Jain University, 18/3, 9th Main Jayanagar 3rd Block, Bangalore 560011, India
 

The chilli crop suffers massive yield loss due to ischolar_main rot caused by Rhizoctonia solani. An increase of 10 × 105 ischolar_main colonizing units/cm was obtained as a result of Bacillus subtilis in vitro ischolar_main colonisation assay post germination. Shelf life studies of the formulations revealed stable population level of the biocontrol agent upto 180th day (30°C - 1.6 × 108; 4°C - 1.9 × 108) in talc and upto 150th day in lignite (30°C - 1.5 × 108; 4°C - 1.3 × 108). Soil, seed, foliar spray and dip treatment methods of B. subtilis and chlorothalonil brought about a considerable enhancement of all biometric parameters and reduced disease incidence compared to the untreated control. In comparison to the untreated control (50 g and 21 g fresh and dry weight, respectively), highest plant fresh weight (76.84 g) and dry weight (34.17 g) was achieved by the Seed application method. Comparison of plant height revealed maximum values 70 cm (soil application) and 77 cm (dip treatment) with B. subtilis application which was analogous to chlorothalonil treatment (56.5 cm with soil application and 70.33 cm with dip treatment) which was considerably superior to the untreated control (58.2 cm with dip treatment and 61 cm with soil application, respectively). Root dip treatment showed considerable increase in ischolar_main length with B. subtilis (33 cm) and chlorothalonil (28.5 cm) when compared to untreated control (15 cm). Growth promotion was better with Root dip application while disease control was achieved better with seed application. A 66% and 84% reduction in incitation of disease was noticed with soil and seed application methods, respectively.

Keywords

Bacillus subtilis, Rhizoctonia solani, bio formulation, shelf life, biocontrol, chilli, pot studies
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  • Biocontrol of Rhizoctonia solani Root Rot of Chilli by Bacillus subtilis formulations under Pot Conditions

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Authors

Ashwini Narasimhan
Department of Microbiology, Center for PG Studies, Jain University, 18/3, 9th Main Jayanagar 3rd Block, Bangalore 560011, India
Srividya Shivakumar
Department of Microbiology, Center for PG Studies, Jain University, 18/3, 9th Main Jayanagar 3rd Block, Bangalore 560011, India

Abstract


The chilli crop suffers massive yield loss due to ischolar_main rot caused by Rhizoctonia solani. An increase of 10 × 105 ischolar_main colonizing units/cm was obtained as a result of Bacillus subtilis in vitro ischolar_main colonisation assay post germination. Shelf life studies of the formulations revealed stable population level of the biocontrol agent upto 180th day (30°C - 1.6 × 108; 4°C - 1.9 × 108) in talc and upto 150th day in lignite (30°C - 1.5 × 108; 4°C - 1.3 × 108). Soil, seed, foliar spray and dip treatment methods of B. subtilis and chlorothalonil brought about a considerable enhancement of all biometric parameters and reduced disease incidence compared to the untreated control. In comparison to the untreated control (50 g and 21 g fresh and dry weight, respectively), highest plant fresh weight (76.84 g) and dry weight (34.17 g) was achieved by the Seed application method. Comparison of plant height revealed maximum values 70 cm (soil application) and 77 cm (dip treatment) with B. subtilis application which was analogous to chlorothalonil treatment (56.5 cm with soil application and 70.33 cm with dip treatment) which was considerably superior to the untreated control (58.2 cm with dip treatment and 61 cm with soil application, respectively). Root dip treatment showed considerable increase in ischolar_main length with B. subtilis (33 cm) and chlorothalonil (28.5 cm) when compared to untreated control (15 cm). Growth promotion was better with Root dip application while disease control was achieved better with seed application. A 66% and 84% reduction in incitation of disease was noticed with soil and seed application methods, respectively.

Keywords


Bacillus subtilis, Rhizoctonia solani, bio formulation, shelf life, biocontrol, chilli, pot studies

References





DOI: https://doi.org/10.18641/jbc%2F30%2F2%2F91995