Journal of Academy of Dental Education

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Identification TIMP-1 and TIMP-2 in Human Radicular Dentine - In Vitro Study


Affiliations
1 Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, India
 

Aim: To identify TIMP – 1 and TIMP – 2 in human radicular dentine using confocal laser scanning microscopy. Materials and Methods: Thirty freshly extracted non carious human single ischolar_mained pre molars were obtained and stored in isotonic saline at -70°C prior to use. All the teeth were decoronated at the CEJ using a diamond. Teeth were divided into 2 groups (Group 1: TIMP-1 analysis n = 15; Group 2: TIMP-2 analysis n = 15). Teeth were sectioned using a hard tissue microtome, mounted and viewed under confocal laser scanning microscopy. Results: TIMP-1 and TIMP-2 were detected in radicular dentine and were seen to be distributed more towards the inner dentine layer closer to the pulp. Conclusion: Due to a shorter half life of TIMP-1 and 2 as compared to the MMP, there is a need to use MMP inhibitors prior to obturation of the ischolar_main canal.

Keywords

Bond Degradation, Collagenolysis, Confocal Laser Scanning Microscopy, Matrix Metalloproteinase, Radicular Dentine, Tissue Inhibitors of Metalloproteinase.
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  • Identification TIMP-1 and TIMP-2 in Human Radicular Dentine - In Vitro Study

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Authors

Kandaswamy Eswar
Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, India
Rubin Mohamed Ismail
Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, India
Hannah Rosaline
Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, India
Nagendrababu Venkateshbabu
Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, India
Deivanayagam Kandaswamy
Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, India

Abstract


Aim: To identify TIMP – 1 and TIMP – 2 in human radicular dentine using confocal laser scanning microscopy. Materials and Methods: Thirty freshly extracted non carious human single ischolar_mained pre molars were obtained and stored in isotonic saline at -70°C prior to use. All the teeth were decoronated at the CEJ using a diamond. Teeth were divided into 2 groups (Group 1: TIMP-1 analysis n = 15; Group 2: TIMP-2 analysis n = 15). Teeth were sectioned using a hard tissue microtome, mounted and viewed under confocal laser scanning microscopy. Results: TIMP-1 and TIMP-2 were detected in radicular dentine and were seen to be distributed more towards the inner dentine layer closer to the pulp. Conclusion: Due to a shorter half life of TIMP-1 and 2 as compared to the MMP, there is a need to use MMP inhibitors prior to obturation of the ischolar_main canal.

Keywords


Bond Degradation, Collagenolysis, Confocal Laser Scanning Microscopy, Matrix Metalloproteinase, Radicular Dentine, Tissue Inhibitors of Metalloproteinase.

References





DOI: https://doi.org/10.18311/jade%2F2014%2F91494