International Journal of Agriculture and Food Science Technology

Ekta Prakash ¹, D. K. Gupta ¹

Affiliations
1 Biochemistry Department, Allahabad University, Allahabad., IN

Abstract

The objective of the present study was to evaluate the in-vitro anti- cancer effects of methanolic extract of jackfruit (Artocarpus integrifolia) latex against MCF-7 human breast cancer cell lines by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) method. The assay provides a convenient, sensitive, quantitative and reliable assay for determining the cytotoxic activity of test sample. The activity was evaluated at different concentration (100 μg, 250 μg, 500μg, 1000μg) of test material. Human breast cancer cell line MCF-7 was taken to see the cytotoxic activity of latex extract. Extract were added in different concentration from 100 μg, 500 μg, 750 μg, 1000 μg to MCF-7 Human breast cancer cell line. Twenty-four-hour exposure to Artocarpus integrifolia extract inhibits cell proliferation as compared with untreated cell. Growth Inhibition was noted 68.3 %, 74.4 %, 77.5 %, 79.9 % against the concentration 100 μg, 500 μg, 750 μg, 1000 μg of test sample respectively. Our results indicate that the plant has a promising anti-cancer activity. Further investigation for active compound present in Artocarpus integrifolia required for cancer management and for the development of new anti-cancer drugs.

Keywords

Jackfruit, MCF-7, Ethanolic Extract of Latex, MTT

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