So-Ra Han ¹, Ji-Ae Jun ¹, Hyun-Suk Yang ², Tae-Jin Oh ¹

Affiliations
1 Department of Pharmaceutical Engineering, Sun Moon University, Asansi, KR
2 Department of Martial Arts and Guard, Sun Moon University, Asansi, KR

Abstract

The present study investigated antioxidant and antimicrobial effects of Hericium erinaceus extracts obtained with acetone, ethyl acetate, and methanol. These extracts were evaluated for antioxidant activities by total polyphenol and flavonoid contents, DPPH radical scavenging activity, and ABTS radical scavenging activity. ABTS radical scavenging activity of the methanol extract showed the highest value similar to that of standard antioxidant, ascorbic acid. The acetone extract and ethyl acetate extract had relatively high total flavonoid contents and DPPH radical scavenging activities. Antimicrobial activities were determined by using disc diffusion method with four strains of Gram-negative bacteria and eleven strains of Gram-positive bacteria including nine oral bacteria. The ethyl acetate extract had antimicrobial activity against Grampositive bacteria, whereas the methanol extract showed inhibitory effect against Gram-negative bacteria. For the microbial activity of oral bacteria, the most of H. erinaceus extracts were effective to inhibit the growth of Streptococcus mutans.

Keywords

Antioxidant Activity, Antimicrobial Activity, Hericium erinaceus

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So-Ra Han ¹, Ki-Hwa Kim ¹, Hyo-Jeong Kim ¹, Sang-Hee Jeong ², Tae-Jin Oh ¹

Affiliations
1 Department of BT-Convergent Pharmaceutical Engineering, SunMoon University, Asansi, KP
2 Department of Dental Hygiene, Gangneung Yeongdong University, Gangneungsi, KP

Abstract

Background/Objectives: To investigate the antibacterial as well as antioxidant activity of Ramariabotrytis. Methods/Statistical analysis: The antibacterial activities of the solvent extractions ((acetone, ethyl acetate, ethanol, and methanol) of R. botrytis were determined against Gram-(+)/Gram-(-) bacteria by disc diffusion method. The antioxidant activities were also checked by measuring free radical scavenging activities (DPPH and ABTS). Findings: All solvent extracts showed an inhibitary effect against E. coli, and especially the ethyl acetate extracts showed higher antibacterial activities against Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. In addition, methanol extracts were effective in DPPH and ABTS activities. The negative correlations were observed between radical scavenging activities of extracts with TPC and TFC. The TPC and TFC were the highest at acetone and ethyl acetate extracts. Improvements/Applications: Ramaria botrytis showed antioxidant and antibacterial activities and so they can be widely used in naturally health food combinations.

Keywords

ABTS, Antibacterial and Antioxidant, DPPH, Ramaria botrytis, TFC, TPC.

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