A. Mungole ¹, R. Awati ², S. Dey ², A. Chaturvedi ², P. Zanwar ²

Affiliations
1 Department of Botany, RTM, Nagpur University, Nagpur 440033, IN
2 SFS Centre for Biotechnology, St. Francis De Sales College, Seminary Hills, Nagpur 06, IN

Abstract

Callus of the leaves, node and bud of Ipomoea obscura (L) was initiated on MS basal media supplemented with various combinations of auxins 2,4- dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid with cytokines kinetin and benzyl amino-purin (BAP). Callus initiation was observed in all media but with varied mass. Highest percentage of callus response was obtained in combination of 2,4-D (0.8 mg/L) with kinetin (0.8mg/L); NAA (0.2mg/L) with BAP(0.2 mg/L); NAA (0.8 mg/L) with kinetin (0.8 mg/L) for explants leaves, node and bud, respectively. The callus was white in 2,4-D- and green in NAA- supplemented MS media. An efficient micropropogation protocol was developed for medicinal plant Ipomoea obscura (L.) by in-vitro culture of nodal part of mature plant. The MS media supplemented with 0.8 mg/L NAA with 0.8 mg/L kinetin induced three shoots per node in an average and was best for axillary bud proliferation.

Keywords

Ipomoea Obscura, Tissue Culture, Medicinal Plant

Full Text