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Adkinsb, S. W.
- Establishment of Mature Axillary Bud Culture of Sugarcane and overcoming Persistent Culture Contamination
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Authors
Affiliations
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, CA
2 Department of Agriculture, The University of Queensland, Brisbane, Qld 4072
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, CN
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, CA
2 Department of Agriculture, The University of Queensland, Brisbane, Qld 4072
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, CN
Source
Indian Journal of Science and Technology, Vol 2, No 1 (2009), Pagination: 18-25Abstract
Several methods to eradicate contaminants from sugarcane mature axillary budculture including bud location/age, surface sterilization and thermal and chemical treatments of buds are examined. A spectacular improvement in the decontamination rate to 95.8 % was obtained when the heat treatment 61 °C for 30 min. was initially applied, followed by either 52 or 57 °C for 30 min, 24 hours apart. The various methods investigated and the successful rates of decontamination of sugarcane axillary buds may have a wider application in other plant tissue culture systems with known persistent contamination.Keywords
Axillary Bud Culture, Tissue Culture Contamination, Disease Free Stock, SugarcaneFull Text
References
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