Barathkumar Sadasivam ¹, Prabu Gajjeraman ¹

Affiliations
1 Department of Biotechnology, Karpagam University, Karpagam Academy of Higher Education, Eachanari, Coimbatore - 641021, Tamil Nadu, IN

Abstract

Background/Objectives: The present study aimed to characterize the role of R2R3-MYB TF gene, ScMYB50/23 from sugarcane during water-deficit stress condition. Methods: The primers specific for ScMYB50/23 were used to isolate the full length cDNA from sugarcane by PCR and characterized their predicted water-deficit stress response in sugarcane tissues (leaf, stem, ischolar_main) by quantitative RT-PCR. The isolated gene was cloned and sequenced for sequence-structure analysis using various computational tools. In order to elucidate its sequence-to-structure-to-function paradigm, comparative modeling, docking and validation were performed using I-TASSER, TFmodeller and 3D footprint platforms, respectively. Findings: The present study provides the first information of stress inducible expression characteristics of the isolated R2R3-MYB TF gene, ScMYB50/23 in sugarcane. PCR amplification showed that the isolated gene was 1080 bp long, which encodes a polypeptide of 359 amino acids with theoretical molecular weight and pI of 39.2 KDa and 9.56, respectively. Comparative modeling and docking of ScMYB50/23 with MYBCORE cis-motif was generated and evaluated by structure quality assessment parameters. Analysis of increased expression pattern of ScMYB50/23 in sugarcane leaf tissue indicates an important role of this gene in water-deficit stress in sugarcane. Application/Improvements: The overall results have demonstrated the explicit role of ScMYB50/23 in conferring water-deficit stress response in sugarcane and have potential to enhance the stress tolerance and crop improvement in plants.

Keywords

Expression, MYB, Stress, Sugarcane, Water-Deficit.

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Hemalatha Balakrishnan ¹, Prabu Gajjeraman ¹, Yasir Umar Pattiwala ¹

Affiliations
1 Department of Biotechnology, Karpagam University (Karpagam Academy of Higher Education), Eachanari, Coimbatore - 641021, Tamil Nadu, IN

Abstract

Background/Objectives: In grass family, sugarcane has been gaining importance due to its role in sugar and biofuel production. We previously reported MYB Transcription Factor (TF) family of genes that regulates almost all biological, physical and biochemical functions in sugarcane. Thus, the present study aimed to characterize an MYB-related Transcription Factor (TF) gene, of ScMYB76 from sugarcane and its expression during water-deficit and salt stress induced condition. Methods: ScMYB76 gene specific primers were designed and used to isolate by PCR from sugarcane leaf tissue. The isolated gene was cloned and sequenced for sequence-structure analysis using various computational tools. To analyze the sequence-to-structure-to-function paradigm, comparative modeling, docking and validation were performed using I-TASSER, TFmodeller and 3D footprint platforms, respectively. The expression pattern during the induced stress condition was analyzed by quantitative RT-PCR. Findings: A cDNA encloding a putative MYB-related TF, ScMYB76 was cloned from sugarcane leaves and consisted of 636 bp Open Reading Frame (ORF) which encodes 212 amino acid length protein with the calculated molecular mass of 23.49 KDa and a pI of 10.2. The deduced ScMYB76 protein was predicted to consist of a single helix-turn-helix MYB-like motif and other residues which are highly conserved among other MYB-related TFs. Comparative modeling and docking of ScMYB76 with MYBCORE cis-motif was generated and evaluated by structure quality assessment parameters. Analysis of expression pattern of ScMYB76 indicates an important function of this gene during abiotic stress in sugarcane. Applications/Improvements: The isolated and analyzed TF gene, ScMYB76 from sugarcane will add a valuable gene resource for crop improvement in regard to abiotic stress.

Keywords

Docking, Expression, MYB-Related, Stress, Sugarcane, Transcription Factor.

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