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Rahumathulla, P. S.
- Molecular Characterization of Vembur Sheep (Ovis aries) of South India Based on Microsatellites
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PDF Views:148
Authors
Affiliations
1 Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, IN
1 Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, IN
Source
Indian Journal of Science and Technology, Vol 2, No 11 (2009), Pagination: 55-58Abstract
The Vembur sheep is a mutton breed, adapted to semi arid habitat and distributed in and around Thoothukudi, Virudhunagar and Tirunelveli districts of Tamilnadu, India. Microsatellite characterization yielded a total of 147 alleles in the sampled population for 25 marker loci under investigation, with a mean of 5.88 ± 0.29 alleles per locus. Number of alleles observed on each locus varied between two and nine. The effective number of alleles observed with a mean of 4.0950 ± 0.23 alleles per locus. The mean PIC value was 0.6905 ± 0.02. The frequency of majority of the loci returned highly significant chi-square values suggesting departure from HWE. The observed heterozygosity (Ho) values varied between 0.1333 and 1.0000, while the expected (He) values ranged from 0.4847 to 0.8537. The mean values were 0.5202 ± 0.04 and 0.7339 ± 0.02 respectively. FIS estimates were negative for three loci indicating more heterozygous nature of the population. The mean value observed was 0.2954 ± 0.31.Keywords
Vembur Sheep, Microsatellites, PIC, FIS, IndiaFull Text
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- Molecular Typing and Mapping of MHC Class II-DRB3 Gene in Indian River Buffaloes (Bubalus bubalis)
Abstract Views :372 |
PDF Views:103
Authors
Affiliations
1 Dept. of Animal Genetics and Breeding, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Chennai - 600 007, IN
1 Dept. of Animal Genetics and Breeding, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Chennai - 600 007, IN
Source
Indian Journal of Science and Technology, Vol 3, No 5 (2010), Pagination: 557-560Abstract
The present study was undertaken with the objectives of characterizing Bubu-MHC loci by PCR and genotyping MHC loci for allelic variation. The PCR product of second exon of the Bubu-MHC-DRB3 gene (304 bp) exhibited genetic polymorphism while digesting with HaeIII enzyme resulting in three restriction fragment patterns in Murrah, four in Surti and three in Murrah graded buffaloes. In all the three genetic groups, the pattern `b' (82, 222 bp) was frequently observed. The restriction fragment analysis with RsaI revealed five patterns in Murrah and three in Surti. The pattern `s' (67, 93, 144 bp) with a frequency of 0.4444 and `l' (67, 237 bp) with a frequency of 0.5000 were observed. Microsatellite typing revealed nine alleles ranging from 160 to 212 bp at the second intron. In Murrah, the allele 190 bp was observed exclusively. In Surti, alleles 192 and 212 bp were observed more frequently. RsaI enzyme revealed more polymorphic patterns of DRB3 than HaeIII. Microsatellite typing provided certain breed-specific alleles. This gene was physically localized to chromosome 2p following tyramide signal amplification in between bands 15-22 using (cDNA probes derived from Bos taurus cattle) fluorescence in situ hybridization.Keywords
Bubu-MHC, PCR-RFLP, FISH, BuffaloesFull Text
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