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Sivananda, N.
- Studies on Adsorption of Diphtheria Toxoid on Aluminium Phosphate Gel
Abstract Views :627 |
PDF Views:129
Authors
N. Sivananda
1,
B. Sundaran
1
Affiliations
1 Pasteur Institute of India, Coonoor, Nilgiris-643 103 Tamilnadu, IN
1 Pasteur Institute of India, Coonoor, Nilgiris-643 103 Tamilnadu, IN
Source
Indian Journal of Science and Technology, Vol 3, No 3 (2010), Pagination: 248-249Abstract
Aluminium compounds are widely used as an adjuvant in Diphtheria, Tetanus, and Pertussis (DPT) group of vaccines. Diphtheria and tetanus toxoids are adsorbed on aluminium phosphate gel and used in the immunization. Generally a plain vaccine is less immunogenic. After adsorption it will have a repositary action. The present study envisages the adsorption rate of diphtheria toxoid on aluminium phosphate gel at different pH (5.0, 6.0, 6.5, 7.0 and 7.5). The main conclusion drawn from the experiments is that the low pH favours high adsorption rate.Keywords
Diphtheria Toxoid, Aluminium Phosphate Gel, Adsorption, VaccineFull Text
References
- Edelman R (1997) Adjuvants for the future 14. In: New generation vaccines. 2nd Edn., Levine MM, Woodrow Gc, Kaper JB & Cobon GS (Eds.). Marcel Dekker Inc, USA.
- Goto N, Kato H, Maeyama J, Eto K and Yoshihara S (1993) Studies on the toxicities of aluminium hydroxide and calcium phosphate as immunological adjuvants for vaccines. Vaccine. 11(9), 914-918.
- Gupta RK and Siber GR (1995) Adjuvants for human vaccines-current status, problems and future prospects. Vaccine. 13(14), 1263-1276.
- Gupta RK (1998) Aluminium compounds as vaccine adjuvants. Adv. Drug Delivery Rev. 32(3), 155-172.
- Lindblad B (2004a) Aluminium adjuvants-in retrospect and prospect. Vaccine. 22, 3658-3668.
- Lindblad B (2004b) Aluminium compounds for use in vaccines. Imm. Cell Biol. 82, 497-505.
- Rao YUB, Ramadevi Venkateshwaran, Rohini Gideon and Vasanth Pandit (1991) Manual for the production and standardization of Diphtheria-Tetanus-Pertusis Vaccine, pp: 12-19, Published by Pasteur Institute of India, Coonoor, Tamilnadu, India.
- Cytotoxic Effect of Diphtheria Toxin in Mammalian Cell Lines
Abstract Views :540 |
PDF Views:200
Authors
Affiliations
1 Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, IN
1 Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, IN
Source
Indian Journal of Science and Technology, Vol 3, No 12 (2010), Pagination: 1177-1179Abstract
Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the bacterial pathogen that causes diphtheria. The present study was focused to find out the toxicological effect of diphtheria toxin in guinea pigs and cell lines like Vero, MA104, HeLa, Murine neuroblastoma-2A, BHK-21, Rhabdomyosarcoma and L20B. Animals inoculated with the toxin at different concentrations showed erythema around the site of inoculation after 48 h. When susceptible cell lines inoculated with similar concentration of toxin showed similar morphological changes with certain minor differences were observed. Diphtheria toxin has the capacity to inhibit protein synthesis in mammalian cell lines and thus causing cell death. The study concluded that Vero, MA104 and Rhabdomyosarcoma cell lines could be used as an alternative to in vivo assay for the toxicology study of diphtheria toxin and enables us free from ethical issues.Keywords
Diphtheria Toxin, Cell Lines, Erythema, Guinea PigsFull Text
References
- Lennox ES and Kaplan AS (1957) Action of diphtheria toxin on cells cultivated in vitro. Proc. Soc. Exp. Biol. Med. 95, 700-702.
- Middlebrook JL and Dorland RB (1997) Response of cultured mammalian cells to the exotoxins of Pseudomonas aeruginosa and Corynebacterium diphtheriae: differential cytotoxicity. Can. J. Microbiol. 23, 183-189.
- Placido-Saousa C and Evans DG (1957) The action of diphtheria toxin on tissue cultures and its neutralization by antitoxin. Br. J. Exp. Pathol. 38, 644- 649.
- Rao YUB, Ramadevi V, Rohini G and Savant Pandit (1991) Manual for the production and standardization of DPT, 32-33.
- Stainer DW and Scholte MJ (1973) The production of high potency diphtheria toxin in submerged culture in relatively simple equipment using a semi synthetic medium. Biotechnol. Bioengg. Symposium. 4, 283- 293.
- Strauss N and Handee ED (1959) The effects of Diphtheria toxin on the metabolism of HeLa cells. J. Exp. Med. 109, 144-162.