Faruq Golam ¹, Kamilatulhusna Zaidi ¹, Arash Nezhadahmadi ¹, Mohamad Osman ²

Affiliations
1 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, MY
2 Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Shah Alam, Selangor, MY

Abstract

The aim of this study was basically to develop new aromatic rice lines through integrated breeding approaches. After screening and selection of superior parental materials, F1s were raised from four different crosses (MRQ50×Gharib, MRQ50×RMB, MRQ50×RTB and MRQ50×E13) and used for haploid production through Anther culture. Phenotypic coefficient of variation % (PCV), genotypic coefficient of variation % (GCV), heritability % (h2), genetic advance (GA) were estimated for yield and yield contributing traits such as for panicle length, grain per panicle, 1000 grain weight and yield per plant. In the cross MRQ50×RMB, the highest PCV was observed in grain per panicle (34.29) followed by yield per plant (31.73), 1000 grain weight (18.44), and panicle length (12.30). The same trends were occurred in cross MRQ50×RTB. The highest GCV was detected in grain per panicle (32.15) followed by yield per plant (30.90), 1000 grain weight (16.67) and panicle length (10.13). However, in the cross MRQ50×RTB, the highest GCV was obtained in yield per plant (37.89) followed by grain per panicle (30.54) and panicle length (9.33). Yield per plant was distinguished as the highest heritable trait in the cross MRQ50×RMB followed by grain per panicle (87.9), 1000 grain weight (81.7), and panicle length (67.8). On the contrary, in the cross MRQ50×RTB, this scenario was a bit different in which yield per plant was detected a highly heritable trait but the trends were different in grain per panicle, 1000 grain weight, and panicle length. Finally, the highest genetic advance was occurred in yield per plant followed by 1000 grain weight, grain per panicle, and panicle length. These trends were equal in both crosses. Study of callus induction ability of all hybrids studies showed that the best performance of callus induction was MRQ50×Gharib and N6 medium was found to be adequate for callus induction.

Keywords

Callus Induction, Haploid, Rice, Hybrid, Genetic Analysis

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