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Liu, Hong
- Differential Expression of PML in 60Co γ-ray and γ-IFN- Induced Apoptosis in B-lymphocytes
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PDF Views:76
Authors
Affiliations
1 Department of Hematology, Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu 226001, CN
2 Department of Radiobiology, Institute for Environmental Sciences, Hachazawa 2-121, Takahoko, Rokkasho, Kamikita, Aomori 139-3212, JP
3 Hiroshima Atomic Bomb Relief Foundation, Kurakake, Asakita-ku, Hiroshima 739-1743, JP
1 Department of Hematology, Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu 226001, CN
2 Department of Radiobiology, Institute for Environmental Sciences, Hachazawa 2-121, Takahoko, Rokkasho, Kamikita, Aomori 139-3212, JP
3 Hiroshima Atomic Bomb Relief Foundation, Kurakake, Asakita-ku, Hiroshima 739-1743, JP
Source
Indian Journal of Science and Technology, Vol 1, No 3 (2008), Pagination: 1-13Abstract
The function of PML in apoptosis was elucidated in human B lymphocyte- derived cell line. For this purpose, the number of the PML focus and the percentage of apoptotic cells were determined periodically with immunofluorescent staining after 60Co γ-ray or γ-interferons (γ-IFN) treatment. On irradiation with 60Co γ-ray or treatment with γ-IFN, PML protein was over-expressed. This was measured from PML foci, which peaked at 72 hr and 24 hr, respectively. The B-cell line also contained a greater proportion of apoptotic cells after the treatments. The strongest induction of apoptosis both by 60Co- γray irradiation and by γ-IFN treatment was observed 24 hr later than the induction of PML expression. In addition, γ- rays-induced apoptosis and PML expression were mediated through caspase-8 but not through caspase-3. However, caspase-8 was involved in γ-IFN-induced PML expression and apoptosis. While caspase-3 is involved solely in PML expression, and partially in apoptosis. These results suggest that 60Co- γ ray irradiation or γ-IFN treatment can induce PML protein expression and apoptosis in the B-cell line as caspase-3 dependently or independently.Keywords
PML Protein, Apoptosis, 60Co-γ Ray, γ-IFN, Caspase ActivationFull Text
References
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- Increased Expression of PML Protein in Lymphocytes Induced by Serum from Patients with Severe Aplastic Anemia
Abstract Views :315 |
PDF Views:81
Authors
Affiliations
1 Department of Developmental Biology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minamu-ku, Hiroshima, JP
2 Department of Radiobiology, Institute for Environmental Sciences, Rokkasho , Aomori, JP
3 Hiroshima Atomic Bomb Relief Foundation, Asakita-ku, Hiroshima, JP
1 Department of Developmental Biology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minamu-ku, Hiroshima, JP
2 Department of Radiobiology, Institute for Environmental Sciences, Rokkasho , Aomori, JP
3 Hiroshima Atomic Bomb Relief Foundation, Asakita-ku, Hiroshima, JP
Source
Indian Journal of Science and Technology, Vol 1, No 4 (2008), Pagination: 1-9Abstract
PML is essential for multiple apoptotic pathways and has an altered expression in human oncogenesis. However, the role of PML in regulating cell growth and apoptosis in aplastic anemia (AA) is still unclear. To investigate the involvement of PML in the pathogenesis of AA, the expression of PML protein and apoptosis in lymphocytes was measured with immunofluorescent staining after 24 hours incubation with serum of healthy individuals (controls, n=10) and patients with severe AA (SAA, n=10). After incubation with serum from SAA patients, PML protein was overexpressed both in peripheral lymphocytes from a normal donor and in a B cell line established from cells of the same individual. The normal lymphocytes contained a greater proportion of apoptotic cells after incubation with serum from SAA patients than serum from controls, which correlated to the serum-induced expression of PML. In addition, the induction of PML expression and apoptosis in lymphocytes by the serum was partly blocked by caspase 8 inhibitor, whereas no significant difference was found before and after caspase 3 inhibitor was added. These results indicate that some components contain of the serum of SAA patients, which was confirmed to γ-IFN by us, acts on lymphocytes not only to upregulate PML protein, but also to selectively activate caspase 8, causing apoptosis. This disregulation may play an important role in the pathogenesis of lymphocytopenia in aplastic anemia.Keywords
Aplastic Anemia, PML Protein, Apoptosis, Caspase 3, Caspase 8Full Text
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