Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sreekumar, G.
- Isolation and Characterization of Probiotic Bacillus Subtilis SK09 from Dairy Effluent
Abstract Views :405 |
PDF Views:192
Authors
Affiliations
1 Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, IN
2 Department of Chemical Engineering, Sathyabama University, Chennai-600119, IN
1 Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, IN
2 Department of Chemical Engineering, Sathyabama University, Chennai-600119, IN
Source
Indian Journal of Science and Technology, Vol 3, No 8 (2010), Pagination: 863-866Abstract
Health promoting microorganisms such as probiotics are recently been used as food additive and therapeutic supplement especially as enhancer of prophylaxis and digestion. Here we report the isolation and characterization of a probiotic bacillus species, with the ability to ferment lactose, from dairy effluent. Biochemical test and 16S rRNA sequencing were done in order to establish the species and strain details. The species was found to be Bacillus subtilis SK09 with a unique ability to ferment lactose. The strain was also subjected to commonly available antibiotics for its antibiotic susceptibility for its probiotic credibility.Keywords
Probiotic Bacteria, Bacillus subtilis, Lactose Intolerance, 16SrRNA SequencingFull Text
References
- Begley M, Hill C and Cormac GM (2006) Bile salt hydrolase activity in probiotics. Appl. Environ. Microbiol. 72 (3), 1729–1738.
- Bruno WJ, Nicholas D Socci and Aaron L Halpern (2000) Weighted neighbor joining: A likelihood-based approach to distance-based phylogeny reconstruction. Mol. Biol. Evol. 17(1), 189-197.
- Conway PL, Gorbach SL and Goldin BR (1987)Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70, 1–12.
- Crittenden RG and Playne MJ (1996) Production, properties and applications of food-grade oligosaccharides. Trend. Food Sci. Technol. 7, 353-361.
- De Vrese M and Stegelmann A (2001) Probioticscompensation for lactase insufficiency. Amer. J. Clinical Nutr. 73, 421S-429S.
- Donkor ON, Henriksson A, Vasiljevic T and Shah NP (2006) Effect of acidification on the activity of probiotics in yoghurt during cold storage. Int. Dairy J. 16 (10), 1181-1189.
- FAO/WHO (2002) Expert consultation: health and nutritional properties of probiotics in food includingpowder milk with live lactic acid bacteria. Cordoba (Argentina) (http://www.who.int/foodsafety/ publications/ fs_management/ probiotics/en/ oppure: http://www.fao.org/es/ESN/food/foodandfood_probioc ons_en.stm)
- Fuller R (1994) Probiotics: an overview. In: Human health: the contribution of microorganisms. Gibson SAW (ed.), Springer-Verlag, NY. pp: 63–73.
- Harley S and Prescott M (2002) Laboratory exercises in microbiology. McGraw Hill. p:58.
- Hyronimus B, Le Marrec C and Urdaci MC (1998) Coagulin, a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4. J. Appl. Microbiol. 85, 42–50.
- Khalid A, Rahim A and Bvong H Lee (1991) Specificity, inhibitory studies and oligosaccharide formation by P-galactosidase from psychrotrophic Bacillus subtilis KL88. J. Dairy. Sci. 74, 1773-1778.
- Kim HS and Gilliland S (1983) L. acidophilus as a dietary adjunct for milk to aid lactose digestion in humans. J. Dairy Sci. 66, 959-966.
- Lin M, Yen CL and Chen SH (1998) Management of lactose maldigestion by consuming milk containing Lactobacilli. Dig. Dis. Sci. 43, 133-137.
- Nicholson W and Setlow P (1990) Molecular biological methods for Bacillus. Harwood C & Cutting S (eds.), John Wiley, NY. pp:391-450.
- Pribila BA, Hertzler SR and Martin BR (2000) Improved lactose digestion and intolerance among African-American adolescent girls fed a dairy-rich diet. J. Amer. Dietetic Assoc. 100(5), 524-528; quiz 529-530.
- Reva ON, Sorokulova IB and Smirnov VV (2001) Simplified technique for identification of the aerobic spore-forming bacteria by phenotype. Int. J. System. Evol. Microbiol. 51, 1361–1371.
- Sneath PHA, Mair NS, Sharpe ME and Holt JG (1986) Endospore-forming gram-positive rods and cocci. In: Bergey’s Manual of Systematic Bacteriology.
- Sreekumar G and Soundarajan K (2010) Bacillus subtilis strain SK09, 16s ribosomal RNA gene sequence. In: Gene Bank, NCBI, freely available at http://www.ncbi.nlm.nih.gov/nuccore/297251625.
- Szilagyi A, Malolepszy P and Yesovitch S (2007) Fructose malabsorption may be gender dependent and fails to show compensation by colonic adaptation. Dig. Dis. Sci. 52, 2999-3004.
- Wayne PA (1997) National committee for clinical laboratory standards (NCCLS) performance standards for antimicrobial disk susceptibility tests, 6th edn. Approved standard M2-A5. Nat. Comm. for Clinical Lab. Standards.
- Weisburg WG, Barns SM, Pelletier DA and Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697–703.
- Wiley EO, Brooks DR, Siegel-Causey D and Funk VA (1991) The compleat cladist: A primer of phylogenetic procedures. Univ. of Kansas Museum of Natural History, Spl. Publ. No. 19. pp:158.