Alexander Pérez-Cordero ¹, Andrea Pérez- Espinosa ², Deimer Vitola -Romero ³

Affiliations
1 Sucre University, Agricultural Sciences, Agricultural Bioprospecting Group. Campus Universitario Puerta Roja, AA 406, Sincelejo - Sucre, CO
2 Sucre University, Health Sciences, Campus Universitario Puerta Blanca, AA 406, Sincelejo - Sucre, CO
3 University of Sucre, Education and Sciences, Bioprospecting Agricultural Group. Campus Universitario Puerta Roja, AA 406, Sincelejo, Sucre, CO

Abstract

Objective: Evaluated resistance capacity in vitro of Bacillus cereus 1DH1LIM at different concentrations of lead in the form of Pb (NO₃)₂. Materials and Statistical Analysis: B. cereus was purified, aliquots of suspensions in log phase were inoculated into minimal medium tris-MMT with different concentrations of lead in the form of Pb (NO₃)₂ and incubated by stirring at 150 rpm at 32 °C for 120 hours; growth was determined by turbidimetry at 600 nm every hour for four days. Siderophore production was determined by growth on médium azurol-S (CAS). Findings: B. cereus 1DH1LIMIt shows statistical difference with respect to adaptation time and concentration of lead metal present in the medium. At concentrations of 100, 150 and 200 ppm of Pb an adaptation phase of 4 hours, with respect to concentrations of 250 to 400 ppm which lasted 7 hours was observed. The highest growth of B. cereus 1DH1LIMIt was observed at 100 ppm, 150 ppm and 200 ppm and less at400. In CAS medium the bacterial culture exhibited siderophore production. Applications: The findings of this study expand the knowledge to use this endophytic bacteria asa biological resource to remedy lead-contaminated environments.

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