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Nezhadahmadi, Arash
- The Application of Irap Markers in the Breeding of Papaya (Carica Papaya L.)
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Authors
Kamaludin Rashid
1,
Rofina Yasmin Othman
2,
Bathusha Shareef Bin Kader Shaik Ali
2,
Yusmin Mohd Yusof
1,
Arash Nezhadahmadi
2
Affiliations
1 Centre for Foundation Studies in Science, University of Malaya, 50603, Kuala Lumpur, MY
2 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, MY
1 Centre for Foundation Studies in Science, University of Malaya, 50603, Kuala Lumpur, MY
2 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, MY
Source
Indian Journal of Science and Technology, Vol 7, No 11 (2014), Pagination: 1720-1728Abstract
Papaya is an important medicinal crop which is used in industries and other healthcare products. This study was conducted to facilitate the selection of Papaya Ring Spot Virus (PRSV) resistant papayas in breeding programs by DNA fingerprinting method. By using Inter-Retrotransposons Amplified Polymorphism molecular markers, 5 out of 45 possible primer combinations (28 IRAP primers combination and RT primers) showed significant polymorphism in DNA banding patterns that determined parental-progeny relations and classified progenies based on their resistance towards PRSV. Primers combination of LTR 6149 and LTR 6149 had the highest degree of polymorphism which was 75% of polymorphic bands. It is observed that only 1 out of 5 combinations of primers demonstrated DNA banding patterns similar to the previous study by Malaysian Agriculture and Research Development Institute (MARDI). Phylogenetic trees were constructed to detect the relation of papaya samples, and those which based on primers combination of LTR 6150 and Nikita showed resistant progenies with resistant parent. Furthermore, progenies from L90 were found to be closely related to the parent Tainung (T5) whilst progenies from L13 and L33 were closely related to another parent, Eksotika (E6). L90 with symptom-free fruits was the most tolerant followed by L33 and L13. So, the best primers in terms of inheritance pattern based on the PRSV resistance trait (LTR 6150 and Nikita primers) were suggested to be further applied in Molecular Assisted Breeding (MAB) or Molecular Assisted Selection (MAS) for papaya breeding programs.Keywords
Breeding, Retrotransposon, IRAP Markers, Carica papaya, Papaya- Potentiality of Roselle and Onion (Allium Cepa) Peel as Raw Materials for Producing Protocatechuic Acid in Tropical Malaysia: A Comparative Study
Abstract Views :327 |
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Authors
Affiliations
1 Plantation and Agro technology, Universiti Teknologi MARA, Shah Alam, Selangor, MY
2 Department of Biotechnology, Faculty of Science, International Islamic University, Kuantan, MY
3 Department of Applied Chemistry, Faculty of Science, International Islamic University, Kuantan, MY
4 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, MY
1 Plantation and Agro technology, Universiti Teknologi MARA, Shah Alam, Selangor, MY
2 Department of Biotechnology, Faculty of Science, International Islamic University, Kuantan, MY
3 Department of Applied Chemistry, Faculty of Science, International Islamic University, Kuantan, MY
4 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, MY
Source
Indian Journal of Science and Technology, Vol 7, No 11 (2014), Pagination: 1847-1851Abstract
Protocatechuic acid from natural sources are widely used in human health. This study focuses on the presence of protocatechuic acid (PCA) in Rosselle (Hibiscus sabdariffa) and onion peel (Allium cepa). Collection of onion peel is difficult and amount is less than 1% in onion. Moreover, Malaysia is importing 100% from overseas. On the contrary, roselle is very common in tropical Malaysia and its calyx can be dried and preserved. Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were used to identify protocathechuic acid (PCA) from the samples. TLC was performed on 20×20 cm Silica gel 60 F254 aluminum sheets as TLC plates with mixtures of water-methanol-formic acid as mobile phase. TLC recorded the presence of protocatechuic acid in both of the samples. HPLC analysis was performed on C-18 in 250×4.6 mm steel column with methanol-water-formic acid in 25:75:0.5 ratio as mobile phase. The flow rate of the mobile phase was 1.0 ml/min and protocatechuic acid was detected at 260 nm of wavelength. The quantities of protocatechuic acid determined by HPLC in rosselle and onion peel were 0.014 mg in 30 g of dried samples extract and 0.25 mg in 30 g of dried extract, respectively. The amount of protocatechuic acid in onion peel extract was eighteen times higher compared to Rosselle extract but considering availability of raw materials in Malaysia, roselle might be used as good source of PCA production with potential economic feasibility.Keywords
Protocatechuic Acid, Roselle, Hibiscus Sabdariffa, Onion Peel- Genetic Analysis of F1 Hybrids Derived from Aromatic (Exotic) × Aromatic (Malaysian) Rice Crosses and Their Callus Induction Performance for Haploid Production
Abstract Views :229 |
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Authors
Affiliations
1 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, MY
2 Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Shah Alam, Selangor, MY
1 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, MY
2 Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Shah Alam, Selangor, MY
Source
Indian Journal of Science and Technology, Vol 7, No 11 (2014), Pagination: 1852-1860Abstract
The aim of this study was basically to develop new aromatic rice lines through integrated breeding approaches. After screening and selection of superior parental materials, F1s were raised from four different crosses (MRQ50×Gharib, MRQ50×RMB, MRQ50×RTB and MRQ50×E13) and used for haploid production through Anther culture. Phenotypic coefficient of variation % (PCV), genotypic coefficient of variation % (GCV), heritability % (h2), genetic advance (GA) were estimated for yield and yield contributing traits such as for panicle length, grain per panicle, 1000 grain weight and yield per plant. In the cross MRQ50×RMB, the highest PCV was observed in grain per panicle (34.29) followed by yield per plant (31.73), 1000 grain weight (18.44), and panicle length (12.30). The same trends were occurred in cross MRQ50×RTB. The highest GCV was detected in grain per panicle (32.15) followed by yield per plant (30.90), 1000 grain weight (16.67) and panicle length (10.13). However, in the cross MRQ50×RTB, the highest GCV was obtained in yield per plant (37.89) followed by grain per panicle (30.54) and panicle length (9.33). Yield per plant was distinguished as the highest heritable trait in the cross MRQ50×RMB followed by grain per panicle (87.9), 1000 grain weight (81.7), and panicle length (67.8). On the contrary, in the cross MRQ50×RTB, this scenario was a bit different in which yield per plant was detected a highly heritable trait but the trends were different in grain per panicle, 1000 grain weight, and panicle length. Finally, the highest genetic advance was occurred in yield per plant followed by 1000 grain weight, grain per panicle, and panicle length. These trends were equal in both crosses. Study of callus induction ability of all hybrids studies showed that the best performance of callus induction was MRQ50×Gharib and N6 medium was found to be adequate for callus induction.Keywords
Callus Induction, Haploid, Rice, Hybrid, Genetic Analysis- Optimization of Media and Cold Pretreatment for Anther Culture Using Japonica/indica and Indica/Indica Hybrids and their Callus Induction Comparisons in Different Rice Crosses
Abstract Views :261 |
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Authors
Golam Faruq
1,
Fathonah Shamsuddin
1,
August Jenifer
1,
Siti Hajar Jusoh
1,
Arash Nezhadahmadi
1,
Nurzulani Khalid
1
Affiliations
1 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, MY
1 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, MY