Mehdi Ghavamizadeh ¹, Ali Mirzaei ²

Affiliations
1 Department of Clinical Biochemistry, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, IR
2 Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, IR

Abstract

Background/Objectives: Medicinal plants play an important role in human health. A large number of plants have claimed for hepatoprotective potential. The aim of this study was to assay the hepatoprotective potential of hydro alcoholic extract of on Carbon tetrachloride induced hepatotoxicity in rats. Materials and methods: Hydro alcoholic extract of Artemisia aucheri at dose of 150, 300 and 600 mg/kg administered on rats for 28 days. The hepatoprotective effect was assessed by serum biochemical tests such as alaine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total protein and albumin. Antioxidant enzymes (superoxide dismutase and catalase), malondialdehyde, F2α-isoprostanes and reduced glutathione were determined along with histopathological studies on liver tissue. Results: Serum levels of total protein, albumin, superoxide dismutase, catalase activities and reduced glutathione were decreased in carbon tetrachloride (toxin) group. However alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, malondialdehyde, F2α-isoprostanes were increased. In treatment groups all biochemical parameters restored toward normal by use of hydroalcoholic extract of A. aucheri. Conclusion: Hydro alcoholic extract of Artemisia aucheri displayed protective potential against carbon tetrachloride induced hepatotoxicity which demonstrated by biochemical tests in tissue homogenate and serum. Also histopathological studies in liver tissue

Keywords

Alanin Amino Transferase, Antioxidant Activity, Aransferase Artemisia Aucheri, Carbon Tetrachloride, F2α- isoprostanes, Hepatoprotective

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Ali Mirzaei ¹, Nooshin Mirzaei ², Alikaram Alamdari ³

Affiliations
1 Medicinal Plant Research Center, Yasuj University of Medical Sciences, Yasuj, IR
2 Medicinal Plant Research Center, Yasuj University of Medical Sciences, Yasuj,, IR
3 Yasuj University of Medical Sciences, Yasuj, IR

Abstract

Background: The biochemical effect of chronic alcohol consumption has been rarely studied although, most of the human population drinks moderate ethanol. The aims of this study was described some biochemical effects induced by moderate ethanol ingestion on rats in four and 12 weeks duration. Methods: Eighteen adult male albino Wistar rats (175 to 210 g) were fed with ethanol (1.6 ml/kg body weight/day) for 4 and 12 weeks. Animals randomly divided into 3 groups (1 to 3) comprised 6 animals each as following: Control group as group (1): Received orally drinking water. Group II: Treated orally with 1.6 g diluted ethanol/ kg body weight daily for 28 successive days. Group III:Treated orally with 1.6 g diluted ethanol/ kg body weight daily for 84 successive days. Blood samples were collected from retero-orbital plexus of animals before to start the ethanol administration (0 week), and at the end of 4 and 12 weeks of ethanol treatment. The serum was used for biochemical tests such as Blood Urea Nitrogen (BUN), Creatinine, Total protein, Albumin, Alanine Amino transferases (ALT), Aspartate Amino transferases (AST), Alkaline Phosphatases (ALP), Glutamyl transpeptidase (GGT) and Nitrite concentration. Reduced Glutathione (GSH), Mallondialdehyde (MDA) content, Catalase, Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPH) activities were estimated on homogenized samples. For Analysis of data paired t-student test was used. Results: Ethanol exposure initially affects liver function followed by renal function. Chronic ethanol ingestion increased hepatocellular enzyme markers such as ALT, AST, ALP, GGT and MDA and nitrate concentration; while decreased total protein, albumin, GSH, ascorbic acid contents and SOD and catalase activities compare to control group. Conclusion: Long-term ethanol exposure induced toxicity which revealed by increase insystemic, local oxidative stress markers and hepatic enzyme indicators.

Keywords

Ethanol, Glutathione, Liver Function, Nitric Oxide, Oxidative Stressss,

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