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Mungole, A.
- In-vitro Callus Induction and Shoot Regeneration in Ipomoea obscura (l.): Potent Indian Medicinal Plant
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Authors
Affiliations
1 Department of Botany, RTM, Nagpur University, Nagpur 440033, IN
2 SFS Centre for Biotechnology, St. Francis De Sales College, Seminary Hills, Nagpur 06, IN
1 Department of Botany, RTM, Nagpur University, Nagpur 440033, IN
2 SFS Centre for Biotechnology, St. Francis De Sales College, Seminary Hills, Nagpur 06, IN
Source
Indian Journal of Science and Technology, Vol 2, No 8 (2009), Pagination: 24-26Abstract
Callus of the leaves, node and bud of Ipomoea obscura (L) was initiated on MS basal media supplemented with various combinations of auxins 2,4- dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid with cytokines kinetin and benzyl amino-purin (BAP). Callus initiation was observed in all media but with varied mass. Highest percentage of callus response was obtained in combination of 2,4-D (0.8 mg/L) with kinetin (0.8mg/L); NAA (0.2mg/L) with BAP(0.2 mg/L); NAA (0.8 mg/L) with kinetin (0.8 mg/L) for explants leaves, node and bud, respectively. The callus was white in 2,4-D- and green in NAA- supplemented MS media. An efficient micropropogation protocol was developed for medicinal plant Ipomoea obscura (L.) by in-vitro culture of nodal part of mature plant. The MS media supplemented with 0.8 mg/L NAA with 0.8 mg/L kinetin induced three shoots per node in an average and was best for axillary bud proliferation.Keywords
Ipomoea Obscura, Tissue Culture, Medicinal PlantFull Text
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Abstract Views :489 |
PDF Views:121
Authors
Affiliations
1 SFS Centre for Biotechnology, St. Francis De Sales College, Seminary Hills, Nagpur-440 006, IN
2 Department of Botany, RTM Nagpur University, Nagpur-440 033, India, IN
3 Department of Botany, RTM Nagpur University, Nagpur-440 033, IN
1 SFS Centre for Biotechnology, St. Francis De Sales College, Seminary Hills, Nagpur-440 006, IN
2 Department of Botany, RTM Nagpur University, Nagpur-440 033, India, IN
3 Department of Botany, RTM Nagpur University, Nagpur-440 033, IN
Source
Indian Journal of Science and Technology, Vol 3, No 6 (2010), Pagination: 679-683Abstract
The present paper deals with in vitro produced phytochemicals of Canscora decurrens (Dalzell) such as alkaloids, flavanoids, phenols, steroids, anthracene glycosides and triterpenoids etc for their antibacterial activities against human pathogenic strains viz. Staphylococcus aureus, Bacillus subtilis, Rhodococci sp., Escherichia coli, Proteus vulgaris, Pseudomonas sp, Salmonella sp. and Bacillus stearothermophilus. The investigation suggests that the plant may be used in therapeutic treatments of gastrointestinal disorders, diarrhoea and skin diseases.Keywords
Phytochemical, Regeneration, Canscora decurrens, Herbal Plant, AntibacterialFull Text
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