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Background/Objectives: Biofunctionalization of nanoparticles is the recent and advanced technology in the fields of nanoscience and biotechnology which produces the environmentally benign and more efficient antimicrobial agents. Therefore, present study reported a simple, convenient and cost effective method of preparation of bioactive antimicrobial agents by the biofunctionalization process of copper oxide nanoparticles with curcuminaniline through a green process method. Methods/Statistical analysis: In this green process two important medicinal quality plant materials, i.e., lemon extract as a reducing agent and turmeric curcumin as stabilizing agent were used to prepare the copper oxide nanoparticles. On other hand, biomaterial curcumin was utilized to prepare the curcuminaniline for the functionalization with copper oxide nanoparticles. The synthesized curcuminaniline, copper oxide nanoparticles and biofunctionalized nanoparticles were characterized by UV- Vis, FT-IR, SEM and TEM techniques. The antibacterial activity of the samples were tested by disc diffusion method against two gram positive bacteria (S.aureus, B. subtilis), two gram negative bacteria (E.coli, S.typhi) and antifungal activity was tested by agar well diffusion method against four funguses (C.albicans, C.lunata, A.niger and T.simii). Findings: The size of synthesized copper oxide nanoparticles was in the ranges around 60-100 nm with dot and needle shaped morphology and biofunctionalized nanoparticles were about 100 nm with spherical shaped morphology. The antimicrobial activities of biofunctionalized copper oxide nanoparticles were observed significant inhibition activity than the non-functionalized nanoparticles and curcuminaniline against S.aureus, B.subtilis, C.lunata and A.niger species. Particularly, the results of antimicrobial activity showed higher efficiency than the standard drugs tested here. Application/Improvements: From this investigation, the green synthesized biofunctionalization method what we have suggested is exposed promise results in the antibacterial and antifungal activity tests. Moreover, biofunctionalized copper oxide nanoparticles are shown better performance in the resisitant of microorganisms than the non-functionalized CONPs and also modified curcumin material. This report helps further for the future scope in the preparation of effective antimicrobial agents.

Keywords

Antimicrobial Activity, Biofunctionalization, Copper Oxide, Curcuminaniline, Green Dynthesis, Nanoparticles
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