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Sabherwal, Suhani
- Azadirachta indica A. Juss. (Neem): Reproductive Studies in Relation to Spermidine Application
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Indian Forester, Vol 136, No 2 (2010), Pagination: 245-256Abstract
The in vivo morpho-physiological responses to spermidine application in Neem (A. indica) were studied during three consecutive annual reproductive flushes from bud development onwards to seed set (prior standardization with endogenous levels). The number of buds and flowers per inflorescence was higher in treated branches as compared to the control. Physiological responses were observed to be polyamine-specific. Year 1 sample protein, total sugar and reducing sugar measured higher following spermidine at 0.2 mM as compared to spermidine at 0.5 mM. The response to year 2 treatments was even better, being significant in 0.2 mM spermidine samples. Year 3 spermidine treatments produced poor flowering response, longer juvenility and low contents of the above compounds as compared to the control. The enzyme assay measuring the activities of invertase, protease, acid- and alkaline phosphatases was consistent with the biochemical responses to the spermidine treatments. In control, a shorter regeneration phase seems to lead to lower productivity in terms of floral inflorescence and fruit set. Therefore, it is suggested that the application of spermidine may regulate the reproductive phase resulting in improved organ/biomolecule production.Keywords
Neem, Azadirachta indica A. Juss., Reproductive Studies, Spermidine Application- Azadirachta indica A. Juss. (Neem) : Protein Profiles during Reproductive Phase
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Indian Forester, Vol 138, No 5 (2012), Pagination: 466-471Abstract
The protein profiles in neem (A. indica) were studied during the annual reproductive flush from bud formation to seed set. Four different zones of activity observed were designated as PRO-I, PRO-II, PRO-III and PRO-IV in the zymogram. It was proposed that decreased protein expression during early pre-anthesis as well as post-pollination fruit-set was due to general inhibition of quantified protein. Besides, specific banding patterns for the various growth stages also point to a definite affect in modulating active metabolism. Hence, decreased protein activity also delayed morphological expression with faint low intensity bands. High frequency bands following flowering and ovary development were point indicators for active protein metabolism and morphogenesis. This resulted in rapid growth as well as healthy fruit-set. The zymogram pattern during such periods traced high mobility, intense zones of activity. The short regeneration phase in neem leads to pharmaceutically significant product of floral inflorescence to fruit set, therefore, it is suggested that protein profiles may be the key in regulating the reproductive phase resulting in improved morpho-physiological characteristics.Keywords
Azadirachta indica, Electrophoresis, Neem, Protein Profiles, Reproduction- Azadirachta indica A. Juss. (NEEM) : Reproductive Studies in Relation to Growth Regulator Treatments
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