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Dobhal, Sneha
- A DNA Extraction Protocol from Leaf Tissues in Quercus Species
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Indian Forester, Vol 139, No 2 (2013), Pagination: 178-179Abstract
No Abstract- An Inexpensive and Rapid Method for Extracting Genomic DNA from Quercus Species
Abstract Views :235 |
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Indian Forester, Vol 139, No 7 (2013), Pagination: 657-659Abstract
No Abstract- Evaluation of Four Different Methods of Genomic DNA Extraction from Quercus leucotricophora
Abstract Views :218 |
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Authors
Sneha Dobhal
1,
H. S. Ginwal
1
Affiliations
1 Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, IN
1 Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, IN
Source
Indian Forester, Vol 140, No 2 (2014), Pagination: 177-179Abstract
The most essential principal in the modern molecular biology is extraction of DNA with a desirable quantity and quality and this achievement could be the main necessity for every genetic study. DNA extraction is obviously difficult because of negative effects obtained from carbohydrates, tannins, polyphenols and proteins. Extraction of DNA from Quercus leucotricophora, was conducted by four different methods. The most value of qualified DNA extracted from young leaf tissue was 629.9 to 1412.1 ng/μl by the method applied by Doyle and Doyle and Stange and his associates with some modifications. Accordingly above method is recommended for DNA extraction from young leaves of Quercus leucotricophora.Keywords
DNA, Extraction, Quercus leucotricophora.References
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- Preliminary Experimental Investigation on Splitting of Bambusa polymorpha
Abstract Views :248 |
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Authors
Affiliations
1 Division of Timber Engineering, Forest Research Institute University, Dehradun, IN
1 Division of Timber Engineering, Forest Research Institute University, Dehradun, IN