Satish Kumar Verma , Santosh Kumar Singh

Abstract

Anticancerous and immunomodulatory properties of Thuja occidentalis extracts were analyzed that showed varied responses. In vitro cytotoxicity (performed by standard SRB assay) was observed against the liver (hep-2) and colon (HT-15) human cancer cell lines. The intraperitoneal injection of methanolic extract (240 mg/kg body weight in Swiss mice), proved to be effective against induced Ehrlich Ascitic Carcinoma. Methanolic extract effectively decreased the cancer cell line counts in peritoneal fluid of EAC mice than the normal control groups of mice. A significant increase in humoral and cell mediated response was observed at high doses of methanolic extract of Thuja occidentalis. Significant alterations in the WBC/RBC count in Ehrlich Ascitic carcinomas bearing mice were observed.

Keywords

Thuja occidentalis, Human Cancer Cell Lines, In vitro Cytotoxicity Test, SRB Assay

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Santosh Kumar Singh , Satish Kumar Verma , Md. Aslam Siddiqui , Devendra Singh , Brij Mohan Sharma

Abstract

Cyanobacteria are a group of diverse Gram-negative organisms that are one of the most primitive photosynthesizes. Attention is drawn towards the alterations in physlogical chances in cyanobacteria exposed to pesticides stress. Synechococcus cultures were exposed to various concentration of the herbicide hexazinone and the tolerant cyanobacteria were used to study the alterations in antioxidant activities, growth and lipid peroxidation levels. All parameters showed a high degree of susceptibility towards high doses of hexazinone. Recovered cyanobacteria showed lower MDA content and super oxide and peroxide radicals. The present findings suggested that high concentration of Hexazinone might modulate the antioxidative system of Synechococcus spp. A significant conclusion can be drawn from the findings that improved tolerance to herbicide stress may be accomplished by increased capacity of antioxidant system of cyanobacteria.

Keywords

Hexazinone, Ascorbate, Lipid Peroxidation, Active Oxygen Species

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