Sarita Arya , Abhinav Kant , Deepa Sharma , I. D. Arya

Abstract

A protocol for the micropropagation of two economically important bamboos, Drepanostachyum falcatum and Bambusa balcooa, which have some problems in their conventional propagation, was developed. Nodal explants with single axillary buds were excised from mother plant and were washed with cetavelon. Initially high incidence of fungal contamination was observed in most cultures of Bambusa balcooa, which was controlled by using Bavistin (1%) for 5-7 minutes followed by surface sterilization for 8 -12 minutes with 0.1% HgCl₂. Liquid MS medium supplemented with 5.0 mg/l 6-Benzyl Amino Purine (BAP) for Drepanostachyum falcatum and 3.0-5.0 mg/l BAP for Bambusa balcooa was found to be the best for axillary bud induction. The multiple shoots were excised from mother explant and further multiplied on MS medium supplemented with defined plant growth regulators. Best shoot multiplication was observed on MS medium supplemented with 3.0 mg/l BAP for Drepanostachyum falcatum and 3.0 mg/l BAP with 0.5 mg/l Kinetin for Bambusa balcooa. The morphogenetic potential of the axillary buds was adversely affected by phenolic exudates especially in Bambusa balcooa. This was overcome by transferring the explant to the fresh medium after every 12-15 days or whenever the medium turned brown. While a regular subculture in every 3-4 weeks increased the multiplication rate in Drepanostachyum falcatum. In-vitro shoots were ischolar_mained when transferred to MS medium supplemented with auxin (IBA, NAA and IAA). The ischolar_mained plantlets of Drepanostachyum falcatum were hardened, acclimatized and successfully transferred to field. However the experiments pertaining to optimal ischolar_maining response and hardening of Bambusa balcooa are in progress.

Keywords

In-vitro, Axillary Bud, Drepanostachyum falcatum, Bambusa balcooa, Nodal
Explant, Shoot Multiplication, In-vitro Rooting

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Richa Satsangi , Sanjay Kalia , I. D. Arya , Sarita Arya

Abstract

No abstract

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Sarita Arya , P. K. Rana , Reetu Sharma , I. D. Arya

Abstract

Micropropagation of Dendrocalamus giganteus through axillary bud culture is described. Multiple shoots were induced from in-vitro culture of nodal shoot segments containing axillary bud. Nodal segments produced axillary shoots in 2-3 weeks on Murashige and Skoog's (MS) medium supplemented with BAP (10-40 μ M). These shoots were excised from mother explants and subcultured on different concentrations of BAP in MS medium. Highest shoot multiplication rate of 5.44 fold was obtained on 20 μ M BAP medium. On MS medium supplemented with Kn only 3-4 fold shoot multiplication was obtained. The addition of BAP (10 μ M) in the kinetin (10 μ M) supplemented medium enhanced the shoot multiplication rate to 6.35 fold. 16-66 % ischolar_mains were formed on excised propagules of 3-5 shoots when transferred to MS medium supplemented with 15-25 μ M IBA. The maximum ischolar_mains per propagule (8.66) and ischolar_maining percentage (90 %) was obtained on MS medium supplemented with 25 μ M IBA + 0.05 μ M BAP. These tissue culture raised plants were successfully hardened and acclimatized and transferred to field condition.

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Sarita Arya , Sushma Sharma

Abstract

Bamboos arc versatile multipurpose forest produce which playa vital role in our domestic economy. Bambusa bambos is the principle source for paper and pulp besides being used for constructional purposes and provides food materials. The conventional methods of its propagation have a lot of problems which restrict its multiplication on a large scale. A tissue culture technology is developed for large scale multiplication of Bambusa bambos which is discussed.

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Ashok Gehlot ¹, Atul Tripathi ², Anuradha Rathore ³, I. D. Arya ², Sarita Arya ²

Affiliations
1 Department of Agriculture and Co-operation, Regional Pesticides Testing Laboratory, Chandigarh, IN
2 Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, Rajasthan, 342001, IN
3 Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, Rajasthan-342001, IN

Abstract

Ailanthus excelsa Roxb. (Simaroubaceae) is a lofty deciduous tree, widely distributed in the country. The seeds, being very light and winged, place early in the first rainy season after the fall of the seed: but the seedlings rarely survive due to the sensitiveness of the seedlings and their intolerance towards heavy weed growth. In the present investigation, Germination experiments were conducted on seeds of Ailanthus excelsa sown in different growth substrates at varying depths. Germination percentage, germination energy and germination values were all greatest when seeds were sown at a depth of 0.5 cm depth and lowest when sown at 1.5 cm depth.

Keywords

Ailanthus excelsa Roxb, Germination Percentage, Germination Energy, Germination Value, Germination Periods, Growth Substrates

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