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Choudhary, Punit
- Collection, Viability and Storage Behaviour of Pollen of some Willow Species/Clones
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Indian Forester, Vol 139, No 8 (2013), Pagination: 706-713Abstract
The genetic improvement of willows through traditional breeding can be facilitated by pollen collection and storage. The female flower receptivity need not be synchronized with pollen shed for breeding. Studies on collection, viability and storage of pollen of commercially important Salix spp. was carried out for implementation of successful breeding programme. The pollen size varied from 20.12 μm to 40.49 μm with clone 131/25 exhibiting maximum size whereas S. babylonica registered minimum size. Germination percentage of the pollen extracted with toluene (Solvent method) was comparatively lower then that of untreated pollen., pollen germination percentage varied between 54.28 to 46.45 per cent in 2009 and 62.88 to 46.88 per cent in 2010. Maximum pollen viability has been recorded at the time of pollen collection. Pollen grains retained higher viability at -20°C even after nine months as compared to pollen stored at 4°C in both the years. Pollen stored at two different temperatures revealed that there was a linear decrease in pollen viability with increase in storage period. The main pollinators found affecting cross pollination among willows were honey bees (Apies spp.), flies, wasps (Polister spp.) and bumble bee (Bambus spp.)Keywords
, Pollen Viability, Storage, Morphology, Pollinators- Molecular Characterization of Promising Willow Clones Using Rapd Markers
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Affiliations
1 Department of Tree Improvement and Genetic Resources, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) 173230, IN
1 Department of Tree Improvement and Genetic Resources, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) 173230, IN
Source
Indian Forester, Vol 140, No 5 (2014), Pagination: 456-461Abstract
Twenty-five promising willow (Salix spp.) clones were selected after nursery screening twice, collected from six countries. Genetic relatedness among the clones was estimated using RAPD markers. Out of 20 primers tested, 16 primers were selected based on the amplification of DNA. A total of 339 bands were scored of which 338 were polymorphic showing high (99.7%) rate of polymorphism. The similarity coefficient ranged from 0.71 to 0.87. These clones were grouped into six different clusters. Clone PN-733 (Salix nigra from New Zealand) appeared to be the most diverse (76 %) from rest of the genotypes on the basis of dendrogram. Maximum similarity (87%) was observed between clones SI-63-007 (S. alba from Italy) and V-99 (Salix alba X S. rubens from Croatia). RAPD analysis proved helpful for estimating the magnitude of genetic diversity at molecular level. The clustering further indicated that the geographic distribution may not be the true index of genetic diversity in willow clones. The marker system was found useful for developing unique fingerprints of three genotypes. On the basis of banding pattern RAPD's were effectively used for molecular characterization of willow clones used in this study.Keywords
RAPD, Willow, Genetic Diversity, Characterization, Polymorphism- Variability Estimate of Growth and Physiological Parameters among Commercially Important Tree Willows
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Authors
Santosh Kumar A. Huse
1,
N. B. Singh
1,
Punit Choudhary
2,
J. P. Sharma
1,
I. K. Thakur
1,
Ratan Kumar Anand
1
Affiliations
1 Department of Tree Improvement and Genetic Resources, Dr. Y. S. Parmar University of Horticulture and forestry Nauni, Solan, H. P., IN
2 Sher- E- Kashmir University of Agricultural Sciences and Technology, Jammu, J&K, IN
1 Department of Tree Improvement and Genetic Resources, Dr. Y. S. Parmar University of Horticulture and forestry Nauni, Solan, H. P., IN
2 Sher- E- Kashmir University of Agricultural Sciences and Technology, Jammu, J&K, IN