P. Maheswara Reddy , L. Prasanthi , P. S. Sudhakar , B. Balakrishna Babu , K. Raja Reddy

Abstract

The results of pooled data analysis of present study on response of Jatropha curcas to varied spacings under irrigated conditions indicated significant variations among the different spacings in parameters of plant growth, seed yield, oil yield and oil content. The plant density at 3×3m spacing showedhigher plant growth as well as seed and oil yield. Wider spacing of 3×3m recorded higher seed yield (2.76 q/ha) followed by 3×2m spacing (2.40 q/ha) and 2×2m (2.25 q/ha) when compared to 4×2m spacings (1.45 q/ha). But, the highest seed oil content of 32.39% was recorded with 3×2m spacing followed by 2×2m spacing (30.87%) and 4×2m spacing (30.48%). While the lowest seed oil content of 28.87% was recorded under wider spacing of 3×3m. However, the highest oil yield was recorded under 3×3m spacing (79.6kg/ha) followed by 3×2m spacing (76.7kg/ha).

Keywords

Jatropha, Spacing, Seed Yield And Oil Content

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L. Prasanthi , B. V. Bhaskara Reddy , K. Rekha Rani , P. Maheswara Reddy , T. Rajeswari , Y. Sivaprasad , K. Raja Reddy

Abstract

A total of 250 random oligonucleotide primers were surveyed to identify sex of Simarouba glauca a tree born oilseed crop. Primer OPA-OS₉₀₀, was found to produce a consistent marker, which differentiated female from both male and andromonoecious sex plants. An identified random amplified polymorphic DNA (RAPD) marker, OPA-OS, linked to male sex in Simarouba was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region (SCAR) primer SCAR(12f/r), which was found promising in male and andromonoecious sex. The marker designed was amplified at a specific site of 915 bp only in male and andromonoecious plants. This would help to identify the sex of tree born oilseed crop Simarouba.

Keywords

Simarouba glauca, Sex Detection, Molecular Marker, RAPD SCAR Marker

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