Ayswarya Radhakrishnan , Manoranjan Bhanja , V. Mohan , Srinivasa Sastry

Abstract

In the present investigation, different forms of P. albus inocula viz., basidiospores, vegetative mycelium and Ca alginate beads were applied to clonal plantlets of Eucalyptus tereticornis in order to determine the effect of each type of inoculum on the growth improvement of the plants. In this study, the treatments with the fungus recorded significantly greater plant height, ischolar_main length, shoot and ischolar_main biomass and collar diameter over uninoculated control plantlets. The maximum growth of plants was recorded in the treatment with pure mycelial culture inoculum, followed by the basidiospore. Least growth was recorded in the calcium alginate treated plants but it had significantly better growth than the untreated control plants.

Keywords

Ectomycorrhizal fungus Pisolithus albus, Inocula, Growth improvement, Eucalyptus tereticornis

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Ayswarya Radhakrishnan , V. Mohan

Abstract

The mycorrhizal symbiosis between plants and fungi is essential for the survival of both partners and it has been suggested that this symbiosis was a prerequisite for the successful colonization by the plants. Among the different mycorrhizal fungi, the ectomycorrhizal fungi are mostly associated with the tree crops particularly conifers and certain angiosperms. Unlike AM fungi, the ectomycorrhizal fungi can be pure cultured and mass produced in synthetic media under laboratory conditions. In the present study an attempt was made to identify the most suitable culture medium for the better growth of three different ectomycorrhizal fungal isolates viz., Pisolithus albus. It was found that all the three isolates performed well in MMN and PHA media. Another study was conducted wherein the three different ectomycorrhizal fungi viz., Laccaria fraterna, Scleroderma sp. and Pisolithus albus were grown in MMN media maintained at seven different pH levels. It was observed that the MMN liquid medium at moderate pH level (5.5 and 6.0) was found suitable for the better growth of all the fungi and significance of the findings is discussed.

Keywords

Ectomycorrhizal Fungi, In-vitro Studies, Culture Medium

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Ayswarya Radhakrishnan , Manoranjan Bhanja , V. Mohan , Srinivasa Sastry

Abstract

In the present investigation, 6 different isolates of P. albus collected from 6 different Eucalyptus plantations zones of Andhra Pradesh viz., Anantapur (isolate-1), Tirupati (isolate-2), Kavali (isolate-3), Paloncha (isolate-4), Tirumala (isolate-5) and Achutapuram (isolate-6) were applied to clonal plantlets of Eucalyptus tereticornis in order to determine the effect of each isolate on the growth improvement of the plants. In this study, the treatments with the fungus recorded significantly greater plant height, ischolar_main length, shoot and ischolar_main biomass and collar diameter over uninoculated (control) plantlets. The maximum growth of plants of Clone 2 was recorded in the treatment with Isolate-5. In Clone 1 better growth performances was observed when inoculated with Isolates 1, 3 and 5. Similarly, Isolates 1, 2 and 5 improved the growth parameters of Clone 3. Least growth was recorded in the plants treated with Isolates 4 and 6 but it had significantly better growth than the untreated control plants.

Keywords

Ectomycorrhizal Fungi, Pisolithus albus, Potential Isolates, Growth Improvement,Eucalyptus tereticornis, Clones

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Ayswarya Radhakrishnan , V. Mohan

Abstract

Thirty two isolates of Azospirillum were isolated from the teak growing areas of Andhra Pradesh, Kerala and Tamil Nadu in South India. The isolates were identified, mass cultured and mixed with carrier material and inoculated to tissue culture raised plantlets of teak in nursery condition to test the efficacy of the isolates on the growth, biomass production and the overall quality of the plantlets. It was found that the isolates ACH-4 (Andhra Pradesh) and KNI-2 (Kerala) were the most potential ones for the growth improvement of the teak plantlets. An attempt was made to determine the synergistic effect between these 2 isolates and it was observed that application of both the isolates put together, not only enhanced the growth but also increased the biomass and the quality of the plantlets.

Keywords

Azospirillum brasilense, Azospirillum lipoferum, Spirillum lipoferum, Tectona grandis, Growth enhancement, South India

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