A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Hadi, Zainab Jaber
- Molecular Detection of Serotype Groups of Listeria monocytogenes Isolated from Gallbladder of Cattle and Sheep in Iraq
Authors
1 Najaf Veterinary Hospital, Najaf Province, IR
2 Department of Clinical and Laboratory Science, College of Pharmacy, University of Al-Qadisiyah, Diwaniyah, IR
3 Department of Microbiology, College of Medicine, University of Kufa, Kufa, IR
4 Department of Microbiology, College of Medicine, Babylon University, Hillah, Babylon Governorate, IR
Source
Veterinary World, Vol 11, No 4 (2018), Pagination: 431-436Abstract
Aim: This study was designed to investigate the occurrence of serotypes of Listeria monocytogenes, an important foodborne pathogen, in gallbladder samples from cattle and sheep.
Materials and Methods: Three hundred samples were collected and screened for the presence of L. monocytogenes. The identification of the isolates was confirmed by API-Listeria system and by the presence of hemolysin (hyl) gene. The isolates were subjected to polymerase chain reaction-based serotype identification with d1 (division 1), d2 (division 2), glt, mama (mismatch amplification mutation assay), and flaA (flagellin protein) genes.
Results: A total of 8 (2.7%) L. monocytogenes were recovered from 6 (4.0%) samples of sheep and 2 (1.3%) samples of cattle. All isolates showed positive results with Hly primers. Four isolates carried d1 gene, did not possess glt gene and harbored mama gene. The serotypes of these isolates were identified as 4a or 4c. The other 4 isolates carried d2 gene, 3 of them were positive with the FlaA primers, and hence, determined to be a 1/2a or 3a serotype, and 1 isolate was determined to be 1/2c or 3c serotype.
Conclusion: This study concluded that the presence of 1/2a serotype in gallbladder samples indicates public health risk through cross-contamination of meat at slaughterhouses.
Keywords
Cattle, Gallbladder, Listeria monocytogenes, Molecular Detection, Sheep.Full Text
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- Molecular Investigation of Plasmid–Mediated Quinolone Resistant Genes among Aminoglycoside-Resistant Uropathogenic Escherichia coli Isolates from Babylon Hospitals, Iraq
Authors
1 Faculty of Medicine, Kufa University, IQ
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 14, No 1 (2020), Pagination: 493-498Abstract
Background: Uropathogenic E coli is the most frequently causes of UTIs in humans ,accountable 75-95 % of UTIs. PMQR have been identified in family Enterobacteriaceae worldwide. The purpose of this study to examine the dissemination of aac(6’)-Ib-cr and PMQR genes in isolates of UPEC.
Method: A cross sectional study of mid-stream urine of the participate patients their approval for direction usage their specimen. The susceptibility test by disk diffusion for UPEC were isolated from urine and screening of quinolone resistant by multiplex PCR for qnrA, qnrB and qnrS genes and monoplex PCR for qunD, qepA and aac(6’)-Ib-cr genes.
Results: A total of 1072 mid-stream urine were collected randomly, overall 132 were identified E. coli, among these 60 aminoglycoside resistant UPEC isolates were screened to the five quinolone antibiotics. The resistance proportion to nalidixic acid and ciprofloxacin were 75.0%, as well as to lomefloxacin, norfloxacin and levofloxacin were 76.7%, 73.3% and 71.7%, respectively. The susceptibility test for antibiotics showed a high incidence of the resistance to the majority of antibiotics class. It was found that 73.3%, 38.3%, and 20.0% of the isolates harbored aac(6’)-Ib-cr, qnrS and qnrB genes, respectively either alone or in combination, while the qnrA, qunD, qepA genes were not detected.
Conclusion: All isolates were identified as multiple antibiotic resistances, only one isolate can be considered as possible PDR and high prevalence of aac(6’)-Ib-cr, qnrS and qnrB genes among isolates.
