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XIONG., YUN
- Evaluation the influence of Aspergillus penicillioides on storage jet fuel and develop a rapid and accurate method for its detection
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Authors
Affiliations
1 Dept. of Oil Application & Management Engineering, Logistical Engineering University, Chongqing 401311, China, Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China, Academic section, Logistics Academy, Beijing, 100000, China., CN
2 Dept. of Oil Application & Management Engineering, Logistical Engineering University, Chongqing 401311, China, Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China, Academic section, Logistics Academy, Beijing, 100000, China, CN
1 Dept. of Oil Application & Management Engineering, Logistical Engineering University, Chongqing 401311, China, Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China, Academic section, Logistics Academy, Beijing, 100000, China., CN
2 Dept. of Oil Application & Management Engineering, Logistical Engineering University, Chongqing 401311, China, Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China, Academic section, Logistics Academy, Beijing, 100000, China, CN
Source
Journal of Mines, Metals and Fuels, Vol 64, No 12 (2016), Pagination: 119-124Abstract
Aspergillus penicillioides is one of the main contaminating fungi in jet fuel result in many damage. This work aims to study the influence of Aspergillus penicillioides on the thermal oxidation stability and corrosivity of jet fuel, meanwhile, developing a repaid and accurate method for detection of Aspergillus penicillioides in jet fuel. The experiment results showed that Aspergillus penicillioides could not only significant weaken the thermal oxidation stability of jet fuel, but also enhance the corrosion ofA3 steel. In this study, a loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick (LFD) method has been developed to detect the 18S ribosomal DNA of Aspergillus penicillioides. The whole detection procedure of LAMP-LFD needed only 90 minutes and detection limit of LAMP-LFD for pure A. penicillioides DNA is 0.82pg/ ¼lwhich was 100 times more sensitive than PCR method. LAMP-LFD could specifically detect A. penicillioides isolates and did not occur non-specific amplification. The LAMP-LFD assay established in this study was proved to be a simpler, more sensitive and accurate method for A. penicillioides genomic DNA detection than PCR and LAMP method, therefore, it is expected to be a new method to detect A. penicillioides in jet fuel.Keywords
Aspergillus penicillioides; thermal oxidation stability; corrosivity; isothermal amplification (LAMP); a lateral flow dipstick (LFD).
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