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Prasad, Y.G.
- Granulovirus of Semilooper, Achaea janata L. (Lepidoptera: Noctuidae): Its Bioefficacy and Safety in Mammalian Toxicity Tests
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Authors
Affiliations
1 Central Research Institute for Dryland Agriculture, Santoshnagar, Hyderabad, 500 059, Andhra Pradesh, IN
1 Central Research Institute for Dryland Agriculture, Santoshnagar, Hyderabad, 500 059, Andhra Pradesh, IN
Source
Journal of Biological Control, Vol 27, No 2 (2013), Pagination: 99–104Abstract
Bio-efficacy of granulovirus (GV) infecting the semilooper, Achaea janata L., a serious defoliating pest of castor bean, Ricinus communis L., was established through leaf disc bioassays. Ovoid occlusion bodies (OB) of A. janata GV (AjGV) measuring 480±20.9 by 240±13.7 nm contained a singly embedded nucleocapsid. Estimated molecular weight of viral genomic DNA obtained with three restriction enzymes was in the range of 124 and 127 kb with a mean of 125.7±0.9 kb. Estimated median lethal concentration (LC50) of AjGV mother culture to second instar larvae was 2.2±0.21×106 OB/ml or 85.4±9.7 OB/mm2 leaf, while for the formulation it was 3.1±0.89×106 OB/ml or 123.3±35.1 OB/mm2 leaf. Kaplan-Meier survival estimate of median lethal time (LT50) for second instars was 7 days at 5×108 OB/ml concentration and 12 days at 5×106 OB/ml. AjGV mother culture and formulation were found to be non-toxic in all the mandatory mammalian toxicity tests. This study fulfills the key data requirements on identification of the AjGV Hyderabad isolate, its bio-efficacy and safety in mammalian toxicity tests.Keywords
Granulovirus, Baculovirus, Bioassay, Achaea janata, Ricinus communis.Full Text
References
- Abdul Kadir HB, Payne CC, Crook NE, Fenlon JS, Winstanley D. 1999. The comparative susceptibility of the diamondback moth, Plutella xylostella and some other major lepidopteran pests of Brassica crops to a range of baculoviruses. Biocontrol Sci Tech. 9: 421–433.
- Allaway GP, Payne CC. 1984. Host range and virulence of five baculoviruses from lepidopterous host. Ann Appl Biol. 105: 29–37.
- Basappa H, Lingappa S. 2002. Management strategies for castor semilooper, Achaea janata (Linn.) (Lepidoptera: Noctuidae) in castor. Indian J Pl Prot. 30: 51–54.
- Bhandari K, Sood P, Mehta PK, Choudhary A. 2010. Effect of granulosis virus infection on food consumption and utilization by Pieris brassicae (Linnaeus). J Biol Control 24: 65–69.
- Bliss C. 1952. The statistics of bioassay. Academic press, New York.
- Burges HD, Croizier G, Huber J. 1980. A review of safety tests on baculoviruses. Entomophaga 25: 329–339. Burges HD, Thomson EM. 1971. Standardization and assay of microbial insecticides. pp. 591–622. In: Burges HD. and Hussey NW. (Eds.), Microbial control of insects and mites. Academic Press, London.
- Damodaram T, Hegde DM. 2010. Oilseeds situation: A statistical compendium. Directorate of Oilseeds Research. Hyderabad, India.
- Eller FJ, Boucias GG, Tumlinson JH. 1988. Interaction between Microplitis croceipes (Hymenoptera: Braconidae) and a nuclear polyhedrosis virus of Heliothis zea (Lepidoptera: Noctuidae). Env Ent. 17: 977–982.
- Evans HF. 1994. Laboratory and field results with viruses for the control of insects. pp. 285-296. In: Hewitt HG, Caseley J, Copping LG, Grayson BT and Tyson D. (Eds.). BCPC Monograph No. 59: Comparing Glasshouse and Field Pesticide Performance II. British Crop Protection Council, Farham, UK.
- Finney DJ. 1971. Probit analysis, 3rd Edition. Cambridge University Press, Cambridge.
- Gaikwad, BB., Bilapate, GG. 1992. Castor semilooper, Achaea janata L. in India – a review. Agri Rev. (Karnal) 13: 51–58.
- Hunter-Fujita FR, Entwistle PF, Evans HF, Crook NE. 1998. Insect viruses and pest management. Wiley, Chichester, UK.
- Lacey LA, Headrick HL, Arthurs SP. 2008a. Effect of temperature on long-term storage of codling moth granulovirus formulations. J Econ Ent. 101: 288–94.
- Lacey LA, Thomson D, Vincent C, Arthurs SP. 2008b. Codling moth granulovirus: a comprehensive review. Biocontrol Sci Tech. 18: 639–663.
- Lakshminarayana M. 1992. Competitive ability of Trichogramma chilonis Ishii and Telenomus proditor Nixon in parasitizing the eggs of castor semilooper (Achaea janata L.). J Oilseeds Res. 9: 351–352.
- Parthasarathy S, Rao AP. 1989. Chemical control of castor semilooper Achaea janata. J Oilseeds Res. 6: 158– 161.
- Payne CC. 1986. Insect pathogenic viruses as pest control agents, Forts Zool. 32: 183–200.
- Prabhakar M, Prasad YG. 2005. Biology and seasonal dynamics of Snellenius maculipennis (Szepligeti) (Hymenoptera: Braconidae) a larval parasitoid of castor semilopper, Achaea janata (Linnaeuus) (Lepidoptea: Noctuidae). J Biol Control 19: 29–34.
- Prasad YG, Prabhakar M, Phanidhara A, Naveen Kumar P. 2010. Development of Achaea janata granulosis virus formulation for use as a viral biopesticide in the management of semilooper on castor. J Oilseeds Res. 27 (special issue): 352–354.
- Prasad YG, Srinivas L, Vimala Devi PS. 2001. A note on granulosis virus infection in Achaea janata Linnaeus. J Oilseeds Res. 18: 285–286.
- SAS Institute 2009. SAS 9.3 for Windows. Cary, NC, USA.
- Singaravelu B, Ramakrishnan N. 1998. Characterization of a granulosis virus from the castor semilooper, Achaea janata L. J Inv Path. 71: 227–235.
- SPSS Inc. 2007. SPSS Base 16.0 for Windows User’s Guide. Chicago, IL, USA.
- Subramanian S, Rabindra RJ, Palaniswamy S, Sathiah N, Rajasekaran B. 2005. Impact of granulovirus infection on susceptibility of Spodoptera litura to insecticides. Biol Control 33: 165–172.
- Tatchel GM. 1981. The effects of granulosis virus infection and temperature on the food consumption of Pieris rapae (Lepidoptera: Pieridae). Biol Control 26: 371–376.
- Van Beek NAM, Wood HA, Hughes PR. 1988. Quantitative aspects of nuclear polyhedrosis virus infections in lepidopterous larvae: the dose-survival time relationship. J Inv Path. 51: 58–63.
- Vimala Devi PS. 1992. Occurrence of mixed infections of granulosis virus and nuclear polyhedrosis virus in castor semilooper Achaea janata Linn. (Lepidoptera: Noctuidae). J Oilseeds Res. 9: 328–330.