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V. Mayekar ¹, M. Pathare ¹, G. Afonso ¹, V. T. Mohite ¹, Y. K. Lahir ¹, P. D. Raut ²

Affiliations
1 Department of Zoology, Thakur College of Science and Commerce, Kandivli (E), Mumbai, IN
2 Department of Environmental Science, Shivaji University, Kolhapur, IN

Abstract

The effects of sub-lethal concentration of nickel on the biochemical composition such as glycogen, protein, cholesterol, lactic acid, pyruvic acid in gills, hepatopancreas, muscles and sugar contents along with haemolymph in female crab, Scylla serrata procured form local fish market in Mumbai (Sassoon Dock) were studied. The female crabs were exposed to sub-lethal dose of nickel LC and LC were 200ppm and 500ppm 0 50 respectively. Among the control set the glycogen contents were found to be the highest in muscles (44.616±0.225 mg/gm wt); protein contents were maximum in gills (66.5232±0.170 mf/gm wt); cholesterol contents were highest in ovary (2.009±0.006 mg/gm wt). The sub-lethal dose of nickel caused decline in the contents of glycogen (1.030±0.0141), protein (11.299±0.055), cholesterol (1.373±0.008) and pyruvic acid (0.440±0.195) in most of the tissues studied. The lactic acid contents increased in all the tissues studied in response to the exposure of nickel. The sugar and lactic acid contents in haemolymph were elevated as a result of exposure whereas the contents of protein, cholesterol and pyruvic acid in haemolymph declined due to the sub-lethal exposure in female crabs.

Keywords

Scylla Serrata, Nickel, Glycogen, Protein, Cholesterol, Lactic Acid, Pyruvic Acid.

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V. T. Mohite ¹, Y. K. Lahir ¹, M. Pathare ¹, S. Agwuocha ¹, U. H. Mane ¹

Affiliations
1 Department of Zoology, Thakur College of Science and Commerce, Kandivli (E), Mumbai 400101, IN

Abstract

This research paper reports on the effects of exposure of green mussel to sub lethal concentrations of cadmium and zinc on GOT, GPT, ACP and ALP activities in the tissues of mantle, gill, hepatopancreas, adductor muscle, siphon, foot and gonad of Perna viridis. Exposure of sub lethal concentration of cadmium for 15 days resulted in the increase of GOT activity in all the tissues and was found to be the maximum in mantle and gonad but declined in hepatopancreas, siphon and foot. 30 days exposure of cadmium caused over all increase in GOT activity but the maximum was found to be in gonad although hepatopancreas and mantle showed higher levels of this activity. The GOT activity increased in all the tissues except in adductor muscle and the maximum in hepatopancreas and gonad as a result of exposure to lower concentration of zinc. Higher concentration of zinc for 30 days resulted in the increase in the activity in all the tissues but being higher in hepatopancreas, gill and gonad. GPT activity due to exposure to lower concentration of cadmium declined in mantle, adductor muscle, siphon, gill and gonad but it showed gentle rise in case of hepatopancreas and foot after 30 days of exposure. The exposure of lower concentration of zinc caused decline in GPT activity in mantle, adductor muscle, siphon, foot and gonad while in gill it increased. The ACP activity increased in all the tissues except in adductor and gonad during 15 days exposure to cadmium while exposure to zinc brought about elevation in all the tissues except gill. The exposure for 30 days resulted in decline in ACP activity in hepatopancreas, mantle and foot. 15 days exposure to cadmium enhanced the ALP activity in all the tissues but not in foot where as 30 days exposure resulted in decline in ALP activity in mantle, gill and adductor muscle. Exposure of zinc caused gradual increase in ALP activity in all the tissues studied. Thus, there is a possibility that Perna viridis can act as an indicator of pollutant contamination due to cadmium and zinc.

Keywords

Green Mussel, Cadmium, Zinc, GOT, GPT, ACP, ALP.

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