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Authors
Affiliations
1 College of Life Science, Shanxi Normal University, Lin Fen, CN
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 8, No 1 (2014), Pagination: 130-135
Abstract
The 7d seedlings of wheat ('ML7113') were exposed to He-Ne laser irradiation (L) with 5mW·mm-2 power density after enhanced UV-B radiation (10.08KJ·m-2·d-1). Total protein were extracted in each treatment group. The total protein were assayed by SDS-PAGE and identified by Western Blot. And analyses the influence of enhanced UV-B radiation-induced wheat leaves lamin B damage repair effects by He-Ne laser.The results shows that enhanced UV-B radiation can damage the lamin B in the seedlings. It may have certain role for repairing the damaged lamin B irradiated by He-Ne laser in the wheat seedlings.In order to futher investigate the effects of UV-B radiation on the relationships between laminB arrangements and 'Partition-bundle division'the dynamic variation of laminB in wheat ischolar_main-tip cells was studied with confocal laser scanning microscopy(CLSM).The results shows that laminB arrays at the periphery of the nucles.
Keywords
Wheat, He-Ne Laser; UV-B Radiation, Lamin B, CLSM
