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Uppada, Vanita
- Cofactor Regeneration - an Important Aspect of Biocatalysis
Abstract Views :279 |
PDF Views:121
Authors
Affiliations
1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400 076, IN
2 Department of Chemistry, Indian Institute of Technology Bombay, Mumbai 400 076, IN
3 Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400 076, IN
1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400 076, IN
2 Department of Chemistry, Indian Institute of Technology Bombay, Mumbai 400 076, IN
3 Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400 076, IN
Source
Current Science, Vol 106, No 7 (2014), Pagination: 946-957Abstract
Biocatalysis has made tremendous advances in the field of synthesis of industrially important products and intermediates. Cofactors are an important part of many enzymes which are involved in biocatalysis. These cofactors are expensive and stoichiometric additions are not economically feasible. This necessitates the in situ cofactor regeneration in biocatalytic processes. Various methods of regeneration of NAD(H)/NADP(H), an important class of cofactors, have been reviewed in this article. We discuss their salient features, suitability for current bioprocesses, drawbacks and scope of improvement.Keywords
Biocatalysis, Biotransformation, Cofactor Regeneration, Enzymes.- Functional Characterization of Alcohol Dehydrogenase from Crabtree-Negative Yeast Komagataella (Pichia) pastoris
Abstract Views :244 |
PDF Views:84
Authors
Affiliations
1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, IN
2 Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, IN
1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, IN
2 Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, IN
Source
Current Science, Vol 111, No 10 (2016), Pagination: 1596-1598Abstract
Alcohol dehydrogenases (ADHs) are fermentative enzymes involved in the inter-conversion of acetaldehyde and ethanol; they are present in multiple copies in Crabtree-positive yeasts. Some have a very narrow substrate range such as acetaldehyde and ethanol, others have a broader substrate range which includes medium and long chain aldehydes and alcohols. They have also been reported in Crabtree-negative yeasts such as Kluyveromyces lactis, Scheffersomyces stipitis, Wickerhamomyces anomalus, Ogataea angusta, Komagataella (Pichia) pastoris, etc.References
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