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Swarupa, V.
- Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
Authors
1 Division of Biotechnology, ICAR - Indian Institute of Horticultural Research, Hesaraghatt Lake Post, Bengaluru - 560089, IN
2 Division of Fruit Crops, ICAR - Indian Institute of Horticultural Research, Bengaluru, IN
Source
Journal of Horticultural Sciences, Vol 9, No 1 (2014), Pagination: 55-60Abstract
Management of the most devasting disease, Fusarium wilt of banana, caused by the fungus Fusarium oxysporum f. sp., cubense, is a challenge to the plant pathologist and the banana grower. Currently, genomics is providing the way for understanding plant defense mechanism, having acquired an important place in crop improvement. To identify the relevant genes and to understand the defense mechanism induced during Fusarium wilt infection, a normalized cDNA library was constructed from infected ischolar_main samples of a tolerant banana genotype, Musa acuminata spp. burmannicoides 'Calcutta-4', by duplex specific nuclease (DSN) based normalization, using the SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction method. Sequencing and analysis of 600 clones revealed 392 non-redundant clones. In all, of 88% of the sequences were annotated using Musa genome database, and the remaining 12% were identified as novel loci not annotated. We observed several resistance genes, ROS scavenging genes and genes involved in ubiquitin-proteosome pathway in this study. These genes may have a possible role against Foc infection. These sequences would enrich the EST data developed against specific stress, which is an indispensable tool for predicting functional genes and understanding the defense mechanism.Keywords
Fusarium oxysporum f.sp. cubense, Banana, cDNA Library, Defense Response, Normalization.- Generalized UPQC System With An Improved Control Method Using PV Array For Reduction Of Harmonics
Authors
Source
International Journal of Innovative Research and Development, Vol 2, No 8 (2013), Pagination:Abstract
Power quality has become an important factor in power systems, for consumer and household appliances with proliferation of various electric and electronic equipment and computer systems. The main causes of a poor power quality are harmonic currents, poor power factor, supply-voltage variations, etc. A technique of achieving both active current distortion compensation, power factor correction and also mitigating the supply-voltage variation of the load side, is compensated by unique device of UPQC. In this paper UPQC with the photovoltaic system is presented. The proposed system consists of a series inverter, a shunt inverter, and a photovoltaic array connected in the DC link through the boost converter. This paper presents a modified synchronous-reference frame (SRF) -based control method to Shunt active filter and instantaneous PQ (IPQ) theory based control technique for series active filtering to compensate power-quality (PQ) problems through a three-phase four-wire unified PQ conditioner (UPQC). The proposed system can improve the power quality at the Point of Common Coupling (PCC) on power distribution systems. The performance of the proposed system was analyzed using MATLAB/SIMULINK.
Keywords
Active power filter (APF), harmonics, Modified phase locked loop (MPLL), power quality(PQ), synchronous reference frame (SRF), unified Power-quality (PQ), conditioner (UPQC), PVArray, Hysteresis controller, Boost Converter- Physiological and Biochemical Changes during Moisture Stress in Banana
Authors
1 Department of Biotechnology, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru (Karnataka), IN