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D. Boopathy ¹, B. Arun Kumar ¹, Devaki Magham ¹, V. Sekar ¹, P. Perumal ¹

Affiliations
1 Department of Pharmaceutical Analysis, J.K.K. Nattraja College of Pharmacy, Komarapalayam- 638 183, Namakkal (D.T), Tamil Nadu, IN

Abstract

A simple, sensitive, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure is developed for simultaneous determination of Rabeprazole sodium and Itopride hydrochloride in Capsule dosage form. The mobile phase used was a combination of methanol: buffer (65:35 v/v) and the pH was found to be 4.30. The detection of the capsule dosage form was carried out at 285 nm and the flow rate employed was 1 mL/min. Linearity was obtained in the concentration range of 2.5 to 15 μg/mL of Rabeprazole sodium and 20 to 120 μg/mL of itopride hydrochloride with a correlation coefficient of 0.998 and 0.996 respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.

Keywords

Rabeprazole Sodium(RS), Itopride Hydrochloride(IH), HPLC.

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V. Sekar ¹, S. K. Bajivali ¹, C. Vanitha ¹, S. Jayaseelan ¹, P. Perumal ¹

Affiliations
1 Department of Pharmaceutical Analysis, J.K.K. Nattraja College of Pharmacy, Komarapalayam- 638 183, Namakkal (D.T), Tamil Nadu, IN

Abstract

In this study reverse phase high performance liquid chromatographic method have been developed and validated for the simultaneous determination of Ambroxol and Levocetirizine in combined pharmaceutical formulation. HPLC separation was achieved with a Phenomenex-Luna, C₁₈ (250x4.6 mm, 5μ) as stationary phase and Sodium Dihydrogen phosphate buffer (pH adjusted to 3 with Ortho phosphoric acid): Methanol (30:70,v/v) as mobile phase , at a flow rate of 1ml/min, UV detection was performed at 230nm. The retention time of Ambroxol and Levocetirizine was found to be 4.2 and 5.6 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and stability studies. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be used for the routine determination of Ambroxol and Levocetirizine in Bulk and in its Pharmaceutical dosage form.

Keywords

Ambroxol, Levocetirizine, RP-HPLC, UV Detection, Methanol.

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D. Boopathy ¹, Devaki Magham ¹, B. Arun Kumar ¹, V. Sekar ¹, P. Perumal ¹

Affiliations
1 Department of Pharmaceutical Analysis, J.K.K. Nattraja College of Pharmacy, Komarapalayam -638 183, Namakkal (DT), Tamil Nadu, IN

Abstract

A Simple and reproducible method for simultaneous estimation of Diethylcarbamazine citrate (DECC) and Cetirizine hydrochloride (CTH) by High performance liquid chromatography (HPLC) in formulation was developed. The analysis was performed with a mobile phase containing Acetonitrile and mixed phosphate buffer (KH₂PO₄ and KH₂PO₄, adjusted to pH - 3.0 with 10% orthophosphoric acid) in the ratio of 60:40(%v/v) at a flow rate of 1ml/min. A phenomenex C₁₈ column (250 × 4.6mm,5μm)was used. The wavelength of DECC is 216nm and Cetirizine is 230nm and from the overlapping spectra a wavelength of 220nm is selected and simultaneous estimation is carried out .The analysis was performed in the linearity range of 24-144 μg/ml for Diethylcarbamazine citrate and 0.8 - 4.8 μg/ml for Cetirizine hydrochloride and the minimum detectable level was 0.62 μg/ml and 0.105 μg/ml.The correlation coefficient was 0.998 and 0.997.Thus the developed and validated chromatographic method for Diethylcarbamazine citrate and Cetirizine hydrochloride is said to be rapid, simple, accurate and cost effective that can be applied for routine analysis in research institution, quality control department in industries and in clinical pharmacokinetic studies.

Keywords

Diethylcarbamazine Citrate, Cetirizine Hydrochloride, RP-HPLC, Simultaneous Estimation, Worm Infestations.

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M. Ramasubramanian ¹, S. Seeralan ², V. Sekar ²

Affiliations
1 Department of Agricultural Extension and Rural Sociology, Agricultural College and Research Institute, Madurai (T.N.), IN
2 Department of Agricultural Extension and Rural Sociology, Agricultural College and Research Institute, Madurai (T.N.), IN

Abstract

Social research and data collection are inseparable. Data collection tool is a weapon in the hand of a social researcher, the sharpness of which decides the accuracy of results. For a very long time we have been using traditional data collection tools such as questionnaire survey, case study method, projective methods etc. In the recent past, participatory rural appraisal (PRA) has been emerging as a data collection tool in social research. Initially, it has been used to draw strategic plan to develop an area by non-Governmental organisations (NGO) and by some Government institutions. But PRA’s role as a data collection tool is recently felt and it is being increasingly used for social science research. In agricultural extension, the researchers used PRA for identification of research priorities, field problem identification, training need assessment, infrastructure facilities availability etc. An exhaustive list of PRA tools recommended for various information required is given which will give a lucid picture of an array of PRA tools available. This article is an epitome of how PRA tools can overcome the difficulties in other data collection tools and the inherent flaws in PRA itself.

Keywords

Rural Appraisal, Data Collection Tool, PRA Tools.

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