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V. Jain ¹, Tripti Jain ², Swarnlata Saraf ¹, S. Saraf ¹

Affiliations
1 University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010, IN
2 Chhattisgarh Food and Drug Administration, Raipur (C.G.) 492 001, IN

Abstract

The Drakshadi gutika is effective for amlapitta (hyperacidity), hrddaha (heart disease), kanthadaha (itching of throat), trsna (thirst), murccha (syncope), agnimandaya (digestive impairment), bhrama (vertigo) and amavata (rheumatism) is official in Ayurveduc formulary in India. Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Ayurvedic medicines/herbal formulations. The Ayurvedic formulation Drakshadi gutika has been prepared as per Ayurvedic formulary of India was estimated HPTLC for its gallic acid content. Three-laboratory batch of Drakshadi gutika were estimated for their gallic acid contents against standard gallic acid solution. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 8.912±0.41w/w in Terminalia belerica, and 0.73±0.63 w/w in vitis vinifera and in three identical laboratory batch of Drakshadi gutika DG-I, DG-II and DG-III, was found to be 3.307±0.52, 3.301±0.63%, 3.314±0.35 w/w respectively. The gallic acid content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine quality control of Drakshadi gutika.

Keywords

Gallic acid, Drakshadi gutika, HPTLC, Fingerprinting, Ayurvedic formulation, Quality control parameter.

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Tripti Jain ¹, Kamlesh Dashora ¹

Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN

Abstract

Hab-e-Azarakhi is an important Unani formulation, is official in Unani Pharmacopoeia (formulary of Unani Medicine). The formulation is dispensed for the tone up the nervous system and liver. It is also useful for increase the appetite. Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Traditional medicines/herbal formulations. Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The present study is an attempt to develop the fingerprint method for Hab-e-Azarakhi by spectrophotometric determination using Piperine as a standard, which is as an important content in formulation. The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 342.6 nm. The UV spectrophotometric analysis, which is a simple, precise, and accurate method that can be considered as one of the quality control method for routine analysis.

Keywords

Hab-e-Azarakhi, Piperine, Ethanol, Spectrophotometer, Unani Formulation.

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Tripti Jain ¹, Kamlesh Dashora ¹

Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN

Abstract

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. A simple, rapid and valid fingerprint method has been first carried out for estimation of piperine in Amlakyadi churna an herbal formulation. Amlakyadi churna is an important ayurvedic formulation, is official in Ayurvedic formulary of India is combination of four reputed herbs, comprised of the fruits Embelica officinalis, Piper longum, Terminalia chebula and ischolar_mains of Plumbago zylenica and Sendha namak. The formulation is dispensed for the treatment of digestive impairment, fever and dyspepsia. The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 342.6 nm.

Keywords

Amlakyadi Churna, Piperine, Ethanol, Spectrophotometer.

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Tripti Jain ¹, V. Jain ², R. Pandey ², A. Vyas ², S. S. Shukla ²

Affiliations
1 Rungta College of Pharmaceutical Sciences and Research, Bhilai, IN
2 Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, IN

Abstract

At the present time, there are a number of non-conventional extraction methods in use that are all, in principle, solid-liquid extractions (SLE) but which introduce some form of additional energy to the process in order to facilitate the transfer of analytes from sample to solvent. These methods include fairly inert, insoluble, and often polymeric material, such as cellulose of plants or fungi and the microbial cell wall. The first step of the extraction is therefore to release and solubilize the smaller secondary metabolites in the matrix, resulting in the initial extract. Forced-flow solid-liquid extraction (FFSLE) techniques, such as medium-pressure solid-liquid extraction (MPSLE) and rotation planar extraction (RPE), in these methods the extraction solvent is forced through the sample bed either by means of pressure or by centrifugal force, thus increasing the efficiency of the extraction process. Even extraction by electrical energy has been studied. The main advantage of these non-conventional methods compared to conventional SLE methods is the increase extraction efficiency, which leads to increased yields and/or shorter extraction times. Indigenous cultures have learnt to exploit the properties of secondary metabolites in many ways, e.g. specific plants or parts of them have been used as poisons, analgesics, stimulants, preservatives, colorants, tanning agents for tanning leather etc. As our understanding of chemistry and other natural sciences has increased, the active chemical compounds of these traditionally used plants have been successfully isolated and identified. There is an increasing trend of using pure compounds instead of crude extracts prepared from plant material, irrespective of their intended use.

Keywords

Extraction Methods, Microwave, Phytoconstituent.

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Tripti Jain ¹, Amber Vyas ², Darshan Dubey ³, Kamlesh Dashora ³, Vishal Jain ²

Affiliations
1 Chhattisgarh Food and Drug Administration, Mahasamund (CG)., IN
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., IN
3 Institute of Pharmacy, Vikram University, Ujjain (MP)., IN

Abstract

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. Fingerprint method for Nilaavaarai Chooranam with TLC Densitometric Methods (HPTLC) using piperine as an internal standard was developed. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation of Nilaavaarai Chooranam and crude drug containing piperine. The concentration of piperine present in raw material was found to be 2.9836±0.497w/w in Piper nigrum and in three identical laboratory batch of Nilaavaarai Chooranam NC-I, NC-II and N-III, was found to be 0.1761 ± 0.384, 0.1759 ± 0.534%, 0.1760 ± 0.019 w/w respectively. The piperine content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Nilaavaarai Chooranam.

Keywords

Nilaavaarai chooranam, Piperine, Sidhha formulation, HPTLC, Standardization.

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