Informatics Publishing Limited

U. S. Mahadeva Rao ¹, San San Oo ¹, Nurul Liana Sabrin ¹, Norsyahira Syazwana Mohd Razali ¹, Khairunnisa Khalid ¹, Nurul Farihah Mohamed Nadzari ¹, Thant Zin ¹

Affiliations
1 Universiti Sultan Zainal Abidin, MY

Abstract

Background: Modifiable Lifestyle Disease (MLD) can give rise to many complications. If left untreated, the disease can affect one's quality of life. MLD can be prevented by modifying our sedentary lifestyle and dietary practice. Introduction: Nowadays, people are aware of the effect of MLDs in their life but the treatment preferences are different among various people. Objective: This study was conducted to identify the treatment seeking behaviour (TSB) among adult populations in East coast Malaysia (Kampung Ladang Sehat, Kuala Terengganu). Method: The survey was conducted by using cross sectional-based questionnaire. The knowledge, attitude and practice among Kampung Ladang Sehat population in East Coast Malaysia with reference to correlation between socio demographic factors and MLD's TSB is studied. Conclusion: The TSB among adult population in Kampung Ladang Sehat is postulated towards down surging according to the correlation between variables used in the study.

Keywords

Modifiable Lifestyle Disease, Treatment Seeking Behaviour, Quality of Life, Dietary Practice.

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Thant Zin ¹, J. Sivakumar ², C. Shanmuga Sundaram ³, U. S. Mahadeva Rao ¹

Affiliations
1 Universiti Sultan ZainalAbidin, Terengganu, MY
2 Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
3 Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN

Abstract

Introduction: <i>In silico</i> analysis of the bioactive compound betuline from the fiddler crab, Uca annulipes and its antimicrobial activities were studied. The fiddler crabs, Uca annulipes were collected from Muttukadu Estuary located on the Kanchipuram District, Tamil Nadu, India, 35 K.M away from Chennai, on the East Coast Road route to Kovalam. Materials and Methods: Fiddler crabs were collected by hand picking method. From the muscle mass of the Crab the bioactive compound was isolated by GC-MS analysis. Molecular docking was performed to identify the protein ligand responsible for the affinity with lung cancer causing tumour cells. Results and Discussion: Hence betuline is the potential lead molecule for the inhibition of lung cancer protein and the most important residues for potential drug target as carbon hydrogen bond, conventional hydrogen bond and Vander Waals interaction. In vitro studies of antimicrobial activities clearly indicated that the different concentrations of betuline bioactive compound have the potential to control the bacteria such as Escherichia coli and Pseudomonas aeruginosa. In vitro studies of anticancer activities also evidently showed the different concentrations of bioactive compound betuline have the potential to control the proliferation of lung cancer cells. Conclusion: Reports are very scanty on this species studied and the reports are very old and hence this present investigation would give latest information on the isolation of bioactive compound for the production of pharmaceutical drugs against the lung cancer. These results will be decisive factor for determining a lead bioactive compound for further drug discovery process for the lung cancer.

Keywords

Betuline, Escherichia coli, Lung Cancer, Pseudomonas aeruginosa and Uca annulipes.

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Thant Zin ¹, J. Sivakumar ², C. Shanmuga Sundaram ³, U. S. Mahadeva Rao ¹

Affiliations
1 Universiti Sultan Zainal Abidin, Terengganu, MY
2 Research Department of Biotechnology, Hindustan College of Arts and Science, Padur, Chennai – 603103, IN
3 Research Department of Microbiology, Hindustan College of Arts and Science, Padur, Chennai – 603103, IN

Abstract

Aim: The present investigation gives a detailed report on the analysis of the bioactive compound isolated from the sand crab Emerita asiatica is having better relation with vasoconstriction. Methods: The sand crab Emerita asiatica were collected from Thiruvidandhai beach located on the Kanchipuram District, Tamil Nadu, India, 41 K.M away from Chennai, on the East Coast Road route to Mahabalipuram. Sand crabs were collected by hand picking method. From the muscle mass of the Crab the organic compound was isolated by GC-MS analysis. Molecular docking was also performed to identify the protein ligand responsible for the constriction of blood vessels. Results: The isolated bioactive compound was identified as phenylephrine, an α1 adrenergic receptor which has the potential to lead the molecule for the vasoconstriction. In vitro studies of antibacterial activities clearly indicated that the different concentrations of phenylephrine have the antibacterial activity against the bacteria such as Klebsiella pneumoniae and Staphylococcus aureus. Summary: The present investigation would give latest information on the isolation of bioactive compound for the production of pharmaceutical drugs from marine organisms. These results will be decisive factor for determining a lead bioactive compound for further drug discovery process for the vasoconstriction.

Keywords

Emerita asiatica, Klebsiella pneumoniae, Phenylephrine, Staphylococcus aureus, Vasoconstriction.

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Kamalakshi Krishnamurthy ¹, Thant Zin ², K. Priyamvatha ³, Mahadeva Rao U.S. ², Suganya M.

Affiliations
1 Additional Director & Director (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai,, IN
2 School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu,, MY
3 Former Student of Women's Christian College, Chennai,, IN

Abstract

Nerium Odorum is an important medicinal plant in India. It is a cardiac glycoside. All parts of the plant are poisonous. Recent studies have thrown light into the fact that it has pharmacological, antibacterial, antifungal, anti-inflammatory, antipyretic, anti-asthmatic and anticancer activities. This study envisages a new developing as well as identification method for Pink/white oleander by Thin Layer Chromatography.

Keywords

Nerium Odorum, Cardiac glycoside, Anti-inflammatory, Anti-asthmatic.

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Kamalakshi Krishnamurthy ¹, Thant Zin ², K. Priyamvatha ³, Mahadeva Rao U. S. ², M. Suganya ⁴

Affiliations
1 Additional Director andDirector (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai, IN
2 School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu, MY
3 Former Student of Women's Christian College, Chennai, IN
4 People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, IN

Abstract

Various new drugs are being introduced in the market. This proves a hectic problem for Toxicologist to identify the exact nature of the drug and its metabolites in the viscera for cases involving death due to drug poisoning. When a case is taken for drug analysis, we have to be more exhaustive in ruling out drugs. For identification of Inderal tablets, a new Thin Layer chromatography system has been developed. After systematic trials, the paper has found out a new solvent system with spray for eluting Inderal tablets.

Keywords

Propranolol – TLC.

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Kamalakshi Krishnamurthy ¹, Thant Zin ², K. Priyamvatha ³, Mahadeva Rao U.S ⁴, Suganya M. ⁵

Affiliations
1 1Additional Director &Director (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai,, IN
2 School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu,, MY
3 Former Student of Women's Christian College, Chennai,, IN
4 School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu, IN
5 People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh,, IN

Abstract

Drug abuse has become more prevalent in India. Smokers mix Ganja (marijuana) along with tobacco in the cigarettes or cigars. Necessity has arisen at this juncture to separately locate Ganja in the admixture of Tobacco and Ganja. The present work envisages a new developing as well as identification method for separating Ganja from Tobacco by Thin Layer chromatography simultaneously in the same plate.

Keywords

Marijuana, Tobacco, Drug abuse, cigarettes

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Kamalakshi Krishnamurthy ¹, Thant Zin ², K. Priyamvatha ³, Mahadeva Rao.U.S ², Suganya M ⁴, K. Mariappan ⁵

Affiliations
1 Additional Director and Director (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai, India ., IN
2 School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu, Malaysia., IN
3 Former Student of Women’s Christian College, Chennai, India., IN
4 People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India., IN
5 Assistant Director (R&D Division), Forensic Sciences Department, Mylapore, Chennai, India., IN

Abstract

Organo-Phosphorous compounds are isolated and located on Thin Layer chromatography by brominating the spotted, eluted plate with subsequent spray of Congo Red. In the above method, the presence of unavoidable coextracts from the visceral matter interferes leading to misconception and false positive results. This paper overcomes the above drawback by finding a new spray in Rhodamine 6G.

Keywords

Rhodamine 6G, Organo-Phosphorous, Congo Red, fluorescent

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