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E. Hema Latha , T. Satyanarayana , A. Ramesh , Y. Durga Prasad , K. V. Routhu , R. L. A. Srinivas

Abstract

Objective: To investigate the hypoglycemic and antihyperglycemic activities of methanolic extract of stem of Argyreia speciosa Sweet (A. speciosa) in normal and alloxan induced diabetic rats. Materials and method: The blood glucose levels were measured at 0 h and 1, 2, 4, 6, 8, 12, 16 and 24 h after the treatment. Oral glucose tolerance test was performed in normal, diabetic control, plant extract treated normal and diabetic groups and tolbutamide also treated normal and diabetic groups. Results: The alcoholic extract of A. speciosa showed significant (P<0.05) dose dependent percentage blood glucose reduction in normal (26.42% at 250 mg/kg, 28.50% at 500 mg/kg and 34.25% at 750 mg/kg body weight) and in diabetic rats (24.72% at 250 mg/kg, 31.10% at 500 mg/kg and 40.47% at 750 mg/ kg body weight) respectively at 8 h. Conclusion: The hypoglycemic and antihyperglycemic effect of A. speciosa was compared with the reference standard drug tolbutamide (40 mg/kg).

Keywords

Argyreia speciosa, Diabetes, Tolbutamide

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T. Satyanarayana ¹

Affiliations
1 Department of Microbiology, University of Delhi South Campus, New Delhi 110 021, IN

Abstract

No Abstract.

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M. Chinna Eswaraiah , T. Satyanarayana

Abstract

Mussaenda erythrophylla (Rubiaceae) is native to western tropical Africa, occasionally seen in gardens and parks as ornamental plant in India. The Hepatoprotective activity of ethyl acetate and methanol extracts of M.erythrophylla (ME) Stem against carbon tetrachloride (CCl4) induced liver damage in Wistar albino rats. Ethyl acetate and methanolic extracts of ME (100,200mg/kg.p.o.), were administerd respectively, Silymarin (25 mg/kg.p.o.) was given as reference standard. The stem extracts were effective in protecting the liver against the injury induced by CCl₄ in animals. This was evident from significant reduction in serum enzyme, SGOT, SGPT, ALP and Total bilirubin (TB).Various pathological changes like centribular necrosis and vacuolization were observed in CCL₄ treated rats, which were significant protective activity in groups treated with ME and silymarin. It was concluded from the study that ethyl acetate and methanolic extracts of ME possess hepatoprotective activity against CCl₄ induced hepatotoxicity in rats.

Keywords

Hepatoprotective, Carbon Tetrachloride, Mussaenda erythrophylla, Silymarin.

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T. Satyanarayana , Kshama Devi , Anjana A. Mathews

Abstract

The hepatoprotective effect of the alcohol extract of Capparis sepiaria Linn. (Capparaceae) stem against carbon tetrachloride (CCl 4 )-induced toxicity was studied in albino rats. The rats were given daily pretreatment with alcohol extract of C. sepiaria (100 mg/kg) and the standard silymarin (25 mg/kg) orally for 7 days. The toxicant used on 7 ^th day was CCl ₄ at a dose of 1.25 ml/kg as 1:1 mixture with olive oil. The extract produced significant (p<0.01) reduction in the elevated levels of aspartate transaminase (AST), alanine transaminase (ALT), total bilirubin (TB) and rise of decreased total protein level when compared with the toxic control.

Keywords

Hepatoprotective, Capparis sepiaria , Carbon Tetrachloride, Silymarin.

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V. Kiran Kumar ¹, N. Appala Raju ², Namratha Rani ², J. V. L. N. Seshagiri Rao ³, T. Satyanarayana ²

Affiliations
1 Dept. of Pharma. Chem., Sree Chaitanya Institute of Pharmaceutical Sciences, LMD Colony, Karimnagar-505527, A.P., IN
2 Dept. of Pharma. Chem., Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road#3, Banjara Hills, Hyderabad-500034, IN
3 University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, A.P., IN

Abstract

A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Irinotecan HCl in tablet dosage form. A X-Terra RP C18, 250×4.6 mm i.d, 5 μm partical size, with mobile phase consisting of Methanol and 0.01 M Ammonium Acetate containing 0.1% formic acid and methanol in the ratio of 50:50 v/v was used. The flow rate was 1 ml/min and the effluents were monitored at 250 nm. The retention time was 4.69 min. The detector response was linear in the concentration of 120-360 mcg/ml. The respective linear regression equation being Y=166582.24x+86439.5. The limit of detection and limit of quantification was 0.06 and 0.18 mcg/ml respectively. The percentage assay of Irinotecan HCl was 99.09%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Irinotecan HCl in bulk drug and in its pharmaceutical dosage form.

Keywords

Irinotecan HCl, RP-HPLC, Estimation, and Tablets.

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V. Kiran Kumar ¹, N. Appala Raju ², Namratha Rani ², J. V. L. N. Seshagiri Rao ³, T. Satyanarayana ²

Affiliations
1 Dept. of Pharma. Chem., Sree Chaitanya Institute of Pharmaceutical Sciences, LMD Colony, Karimnagar-505527, AP, IN
2 Dept. of Pharma. Chem., Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road 3, Banjara Hills, Hyderabad-500034, IN
3 University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, AP, IN

Abstract

A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Paclitaxel in Parenterals dosage form. A Unison US C18, 250×4.6 mm i.d, 5 μm partical size, with mobile phase consisting of Methanol and 0.02 M potassium dihydrogen phosphate in water (pH 2.5 adjusted with o-phosphoric acid) in the ratio of 80:20 v/v was used. The flow rate was 1 ml/min and the effluents were monitored at 225 nm. The retention time was 4.978 min. The detector response was linear in the concentration of 15-180 mcg/ml. The respective linear regression equation being Y=46411.83x+29.2578. The limit of detection and limit of quantification was 0.03 and 0.09 mcg/ml respectively. The percentage assay of Paclitaxel was 99.09%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Paclitaxel in bulk drug and in its pharmaceutical dosage form.

Keywords

Paclitaxel, RP-HPLC, Estimation, and Parenteral.

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Beedha Saraswathi ¹, Sk. Shabana ¹, D. Ramya Sri ¹, D. Anantha Lakshmi ¹, M. Shivaji ¹, T. Satyanarayana ¹

Affiliations
1 Department of Pharmaceutics, Mother Teresa Pharmacy College, Sathupally-507303, Khammam Dist, IN

Abstract

Topical drug delivery system (TDDS) facilitates the passage of therapeutic quantities of drug substance through the skin and into the general circulation for their systemic effects. Although having plenty of advantages over other routes of administration topical drug delivery system is having certain limitations including hydrophilic drugs cannot easily penetrate across skin, to overcome this problem drug made into sufficient lipophilic or lipophilic drugs are sued along with certain penetration enhancers which help to achieve desired results. Tramadol is a narcotic analgesic proposed for moderate to severe pain. It may be habituating. Tramadol and its metabolites are excreted primarily in the urine with observed plasma half-lives of 6.3 and 7.4 hours for tramadol and M1, respectively. Approximately 30% of the dose is excreted in the urine as unchanged drug, whereas 60% of the dose is excreted as metabolites. On this contest, emulgel was formulated using carbopol 934 and HPMC K15M, liquid paraffin as oil phase, emulsifying agents like tween 20 and span 20 and propylene glycol as permeation enhancers. On basis of quality of emulgel produces total eight formulations F1 to F8 were selected. They were evaluated for physical appearance, pH, rheological study, drug content and in-vitro drug permeation study, FTIR and globule size determination. The skin irritation test was performed on rabbit’s skin using best formulation F7. Thus, the formulated emulgel had a distinct advantage over existing conventional dosage form in that the drug permeation was found to be rapid across the skin and hence the increased therapeutic response by bypassing 1st pass metabolism and with no gastro intestinal bleeding and also patient compliance.

Keywords

Emulsion, Gel, Transdemal Drug Delivery Systems, Tramadol, In-Vitro Drug Permeation Study.

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Beedha Saraswathi ¹, T. Satyanarayana ¹, K. Mounika ¹, G. Swathi ¹, K. Sravika ¹, M. Mohan Krishna ¹

Affiliations
1 Department of Pharmaceutics, Mother Teresa Pharmacy College, Sathupally, Khammam Dist., 507303, IN

Abstract

Transdermal drug delivery leads direct access to the systemic circulation through the skin which bypasses drugs from the hepatic first pass metabolism leading to increase bioavailability. Tramadol HCl has been selected as model drug because it has low bioavailability. It exhibit all physicochemical characteristics required for the transdermal patch. Transdermal patches of Tramadol HCl were prepared by solvent casting method using different polymers i.e. HPMCK4M, HPMCK15M, HPMCE5.Propyleneglycol was used as plasticizer and methanol was used to dissolve the drug. Water was used as solvent to dissolve the polymer. The prepared formulations were evaluated for drug content uniformity, in vitro diffusion study, thickness, tensile strength, moisture content, folding endurances etc. Amongst all formulation, formulation F3 had more desirable characteristic and shows 88.36% drug release in 12hr. Release kinetic can be described by Higuchi model with anomalous diffusion as a release mechanism. The Transdermal patch formulated from HPMCK4M, HPMCK15M and HPMCE5 showed satisfactory physicochemical properties. The ratios of hydrophilic polymers HPMCK4M, HPMCK15M and HPMCE5 good moisture content property, good tensile strength, folding endurances and in-vitro drug release. So, it can be concluded that such a matrix type patches of HPMCK4M, HPMC K15M and HPMCE5 could be a good carrier in transdermal delivery of Tramadol HCl. FTIR studies showed there were no incompatibilities between drug and other excipients

Keywords

Transdermal Patch, Tramadol HCl, HPMC.

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