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Radha, T. K.
- Soil and Plant Analysis - A Strategic Tool to Diagnose Micronutrient Imbalance in Lime and Sapota Orchard in Tablelands of Chambal Ravine Region of India
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Authors
Affiliations
1 ICAR-Indian Institute of Soil Water Conservation, RC, Kota, IN
2 ICAR-Indina Institute of Soil Science, Bhopal, IN
3 ICAR-Indian Institute of Horticulture Research, Bengaluru - 560 089, IN
1 ICAR-Indian Institute of Soil Water Conservation, RC, Kota, IN
2 ICAR-Indina Institute of Soil Science, Bhopal, IN
3 ICAR-Indian Institute of Horticulture Research, Bengaluru - 560 089, IN
Source
Journal of Horticultural Sciences, Vol 15, No 1 (2020), Pagination: 72-80Abstract
Micronutrient imbalance in lime and sapota fruit crops result in unstable fruit yield, fruit shedding and degrade quality of the produce. A study was therefore conducted to evaluate micronutrient statusoflime and sapota orchard by analysing soil and plant samples. Soil samples were collected from surface (0-15cm) and sub-surface (15-30cm)depth representing whole orchard. At the same time, plant samples including 35-40 each for leaves and petiole samples each from lime and sapota field was also collected.Available micronutrients from soil samples were extracted using diethylenetriaminepenta acetic acid (DTPA) and it was in the order of manganese (Mn)> iron (Fe)> zinc (Zn)> copper (Cu) in both lime and sapota plantations. DTPA- extractable Zn and Cu showed low status, marginal status of Fe and sufficient level of Mn in soils of sapota plantations. In plant analysis, high concentration of Cu (869 mg kg-1) and Zn (411mg kg-1) was observed in lime leaves; however, in sapota crop Cu and Zn content was 8.25mg kg-1 and 16.7mg kg-1 respectively. Similarly, Fe and Mn content of lime leaves was 197 and 43 mg kg-1 which was slightly higher than sapota leaves that recorded 128 and 49mg kg-1 of Fe and Zn respectively. In sapota plants, higher Mn and Cu concentration in leaf resulted in Zn deficiency symptoms such as shortened internodes or rosette disorders of sapota plants. Thus, correcting micronutrient deficiency is pre-requisite for qualitative and quantitative fruit production in tablelands of India.Keywords
Copper, Iron, Leaf Analysis, Manganese, Micronutrient Deficiency, Sapota, Zinc.References
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- A New Modified Nutrient Media for Cultivation of Actinobacteria
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PDF Views:33
Authors
Affiliations
1 Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, IN
1 Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, IN
Source
Current Science, Vol 120, No 4 (2021), Pagination: 718-722Abstract
The growth of Actinobacteria was evaluated in a new modified nutrient agar media (MNA) supplemented with 1% sodium chloride (NaCl) and compared with the universally accepted media which are under regular use. Thirteen different Actinobacterial isolates were evaluated for their growth in both solid and liquid media in MNA along with four regularly used media ISP-2, kenknight, starch casein and nutrient media. Results indicated that eight isolates (Streptomyces bulli, Streptomyces griseorubens, Streptomyces viridobrunneus, Streptomyces A3, Streptomyces A7, Streptomyces A11, Streptomyces A12 and Streptomyces A13), out of thirteen isolates could grow significantly faster on MNA compared with other solid agar media. Similarly, growth in liquid media recorded by optical density (OD) indicated that three isolates (S. griseorubens, S. viridobrunneus and A7) showed significantly higher OD followed by ISP-2, nutrient, starch casein and kenknight broth after seven days of incubation. All the isolates except S. viridobrunneus and Streptomyces A12 could tolerate NaCl concentration upto 6% in liquid modified nutrient broth. The present study showed that Actinobacteria can be successfully grown faster with full pigmentation at 1% NaCl concentration and they can tolerate up to 5% NaCl concentration, in the new proposed MNA media.Keywords
Actinobacteria, Modified Nutrient Agar Media, Optical Density, Sodium Chloride.References
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