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Dobhal, Sneha
- A DNA Extraction Protocol from Leaf Tissues in Quercus Species
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Indian Forester, Vol 139, No 2 (2013), Pagination: 178-179Abstract
No Abstract
- An Inexpensive and Rapid Method for Extracting Genomic DNA from Quercus Species
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Indian Forester, Vol 139, No 7 (2013), Pagination: 657-659Abstract
No Abstract- Evaluation of Four Different Methods of Genomic DNA Extraction from Quercus leucotricophora
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Authors
Sneha Dobhal
1,
H. S. Ginwal
1
Affiliations
1 Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, IN
1 Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, IN
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Indian Forester, Vol 140, No 2 (2014), Pagination: 177-179Abstract
The most essential principal in the modern molecular biology is extraction of DNA with a desirable quantity and quality and this achievement could be the main necessity for every genetic study. DNA extraction is obviously difficult because of negative effects obtained from carbohydrates, tannins, polyphenols and proteins. Extraction of DNA from Quercus leucotricophora, was conducted by four different methods. The most value of qualified DNA extracted from young leaf tissue was 629.9 to 1412.1 ng/μl by the method applied by Doyle and Doyle and Stange and his associates with some modifications. Accordingly above method is recommended for DNA extraction from young leaves of Quercus leucotricophora.Keywords
DNA, Extraction, Quercus leucotricophora.References
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- Laura, M.; Benedetti, L. De.; Bruna, S.; Burchi, G.; Berio, T.; Giovannini, A. and Ruffoni, B.(2003). Cyclamen persicum mill: Somatic embryogenesis and RAPD analysis of embryogenic callus. Acta Hort., (ISHS) 625: 137–44
- Lodhi, M.A.; Ye, G.N.; Weeden, N.F. and Reisch, B.I. (1998). A simple and efficient method DNA extraction from grapevine caltivars and vitis species. Plant Mol. Biol. Rep., 12: 6–13.
- Naderi, R.; Khalighi, A.; Arzani, K. and Babalar, M. (2000). Effects of storage temperature, chemical treatment, harvesting stage and genotype on vase life of cut flowers of cyclamen (Cyclamen persicum Mill.). Seedand Pl. J., (SPII), 16: 240–51
- Porebski, S.; Bailey L. and Boum; B.R. (1997). Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep., 15: 8–15.
- Stange, C.; Prehn, D. and Johnson, P.A. (1998). Isolation of Pinus radiata genomic DNA suitable for RAPD analysis. Plant Molecular Biology Reporter, 16:1-8.
- Zhang, J.; McDonald, M.B. and Sweeney, P.M. (1997). Testing for genetic purity Petunia and Cyclamen seed using random amplified polymorphic DNA markers. J. Amer. Soc. Hort. Sci., 32: 246–7.
- Preliminary Experimental Investigation on Splitting of Bambusa polymorpha
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Authors
Affiliations
1 Division of Timber Engineering, Forest Research Institute University, Dehradun, IN
1 Division of Timber Engineering, Forest Research Institute University, Dehradun, IN
Source
Indian Forester, Vol 141, No 6 (2015), Pagination: 642-647Abstract
The study was investigated the splitting failure of Bambusa polymorpha through two testing methods: fracture test and edge bearing test. This is mainly done with an aim to generate preliminary data for studying splitting behaviour of Bambusa polymorpha. The fracture test appears to yield both reliable and accurate results, but so far only one geometry has been tested for split pin test. It has yet to be shown whether a change in the pin diameter, the culm diameter, or the size of the initial crack will have any effect on the stress intensity obtained. It is further proposed that selecting a pin diameter similar to that used for bolted connections may help to develop appropriate values for design. In edge bearing test there is no significant differences were found in considering specimen with different length. As the engineering interest in bamboo is recent, there are many areas still requiring further investigation. The fracture test appears to yield both reliable and accurate results, but so far only one geometry has been tested. In edge bearing test there is no significant differences were found in considering specimen with different length.Keywords
Bambusa polymorpha, Splitting.- Production and Conservation of Medicinal Plants in Understorey of Degraded Chir Pine Forests Using Sustainable Techniques
Abstract Views :271 |
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Authors
Affiliations
1 Uttarakhand Forest Department, Dehradun 248 001, IN
2 ICAR-Indian Institute of Soil and Water Conservation, RC, Vasad 388 306, IN
3 College of Forestry, Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, IN
4 Department of Forestry, Sarguja Vishwavidayalaya, Ambikapur 497 001, IN
5 College of Forestry, Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, IN
1 Uttarakhand Forest Department, Dehradun 248 001, IN
2 ICAR-Indian Institute of Soil and Water Conservation, RC, Vasad 388 306, IN
3 College of Forestry, Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, IN
4 Department of Forestry, Sarguja Vishwavidayalaya, Ambikapur 497 001, IN
5 College of Forestry, Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, IN
Source
Current Science, Vol 112, No 12 (2017), Pagination: 2386-2391Abstract
In general, little emphasis is given on the production of medicinal plant species for sustainable harvest and conservation in the understorey of degraded forests. For instance, forests like chir pine which are not managed to grow any medicinal plants, offer an opportunity to produce and conserve native medicinal plants. This article highlights a study focusing on the production of medicinal plants in association with Chir pine forests, involving sustainable management techniques like minimum tillage and selecting suitable aspects for the sustainable harvest and conservation of plants under the canopy of trees. The medicinal plants were grown on three topographical aspects - Northern, North-Western and Western by adopting three tillage depths, viz. minimum (0 cm), medium (up to 10 cm) and deep tillage (up to 15 cm), in open and below tree canopy conditions. From yield and economic point of view, Andrographis paniculata, Mucuna pruriens, Solanum khasianum and Spilanthes acmella were found to be better yielding and more remunerative in comparison to Withania somnifera, Cymbopogon nardus and Ocimum basilicum, when grown outside the tree canopy adopting deep tillage on the Western aspect in Chir pine forests. The study can also be applicable to the other parts of the country to produce and conserve native medicinal plant species using sustainable management techniques like minimum tillage in blank patches or understorey of the degraded forests.Keywords
Chir Pine, Degraded Forest, Medicinal Plants, Production and Conservation, Sustainable Management.Full Text
References
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- Sanwal, C. S., Bhardwaj, S. D., Sushma, K., Lone, R. A., Pant, K. S. and Khan, P. A., Effect of variation of aspect and frequency of tillage depth on growth and yield attributes of Ashwagandha (Withania somnifera) in mid hills of western Himalayas. Med. Plants, 2013, 5, 1–5.
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- Morphological Characterization and Identification of Populus deltoides Bartr. Crosses
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Authors
Affiliations
1 Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), IN
2 ICAR-IISWC, RC, Vasad, Anand (Gujarat), IN
1 Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), IN
2 ICAR-IISWC, RC, Vasad, Anand (Gujarat), IN
Source
International Journal of Forestry and Crop Improvement, Vol 8, No 1 (2017), Pagination: 24-29Abstract
Leaves are of fundamental importance to plants, representing their facility to generate power and are the sensing units of plants towards the environment. In this study, an attempt was made to characterize and compare the variations of leaf, stem and petiole morphology of various Populus deltoides Bartr. crosses. In order to achieve these objectives, twelve crosses of P. deltoides Bartr. were evaluated for different parameters. On the basis of various morphological characteristics the results revealed that each cross has a distinct color pattern of leaves. Different colors observed in these crosses varied from yellow green to green. Distinct nature of the stem altitude was found. Anthocyanin distribution of leaves was also found. In this study, the morphological traits of leaves and petiole provided discriminatory grounds for separating various populations of P. deltoides Bartr. crosses. Character of stem studies indicates that different crosses vary considerably with regard to shape, colour and nature.Keywords
Populus deltoides Bartr., Leaves, Morphology, Petiole.References
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