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Shukla, Shruti
- Isolation, Identification, Partial Purification, Optimization and Characterization of Alkaline Protease From Neisseria flavescence
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Science, ALLAHABAD (U.P.), IN
Source
Asian Journal of Bio Science, Vol 10, No 1 (2015), Pagination: 57-64Abstract
Microbial alkaline proteases are among the important hydrolytic enzyme and have been used extensively since the advent of enzymology. Bacterial extracellular alkaline proteases are of great importance due to its wide spectrum applications in detergent industries, bioremediation, food industries, and leather processing and bio-film degradation. From the various niches eighteen isolates were screened for alkaline protease production, out of which four isolates showed efficient alkaline protease production. Out of four bacterial species one of the isolate i.e. N. flavescence showed significant enzyme activity. Optimization of the pH and temperature conditions for enzyme production was determined and was found to be 7.0 and 60°C, respectively. Optimization of carbon, nitrogen sources and metal ions for enzyme production were determined and was found to be 4.64U/ml for sucrose, 0.91U/ ml for gelatin and 0.21U/ml for zinc chloride for the isolates. The yield of alkaline protease was inhibited by copper sulphate. Enzyme activity was assayed using tyrosine-casein method. The purified enzyme preparations of having enzyme activity 0.26U/ ml was also excellent in destaining of ink colour. The molecular weight of different bands of alkaline protease fromN. flavescence ranged from 25kD to 83kD.
Keywords
Neisseria flavescence, Alkaline protease, SDS, Sucrose, Gelatin, Zinc chloride, Copper sulphate.
- Intra-Household Gender Inequality in Food Intakes and Efficacy of Food Intakes and Patterns
Authors
1 The Institute of Applied Statistics and Development Studies, C-36, Near Hans Bhakti Dham, Katnla Nehru Nagar, Ring Road, Lucknow - 226022, IN
Source
The Indian Journal of Nutrition and Dietetics, Vol 47, No 3 (2010), Pagination: 94-103Abstract
Institute of Applied Statistics and Development Studies (lASDS), Lucknow conducted o survey based study' "Gender Bias in Intra-Household Food Consumption: Dietary intake and Pattern' for Indian Council for Medical Research (ICMR) to assess the difference in the dietary lntakes of different age-sex groups.- Isolation, Partial Purification, Product Formation and Characterization of β- Glucosidase from Roots of Hordeum vulgare L.
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 52-55Abstract
β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.
Keywords
Hordeum vulgare, β-Glucosidase, Purification, Characterization.- Assessment of Physico-Chemical Parameters of Sagar Lake, India
Authors
1 Department of Botany, Lab of Microbiology, Dr. H. S. Gour Univ., Sagar (M.P.), IN