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J. Anbu , R. Vasuki , P. Shanmugasundaram , Shiny George , R. Sujatha , M. Vijey Aanandhi

Abstract

The methanolic extract of the Citrus sinensis peel was screened for antidiarrhoeal activity using animal models against castor oil induced diarrhoea. The parameters evaluated are small intestine secretion by enteropooling assay, rate of defecation and small intestine transit. The methanolic extract showed significant activity (p<0.05) against castor oil induced diarrhoea and interluminal accumulation of fluid. It also reduced gastrointestinal motility after charcoal meal administration in albino mice. The results indicates that the action of Citrus sinensis peel extract (CSPE) could be through a combination of inhibition of elevated prostaglandin biosynthesis and reduced propulsive movement of the small intestine.

Keywords

Citrus Sinensis, Anti Diarrhoeal, Albino Mice, Castor Oil and Charcoal Meal

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Meena Chandran ¹, Shiny George ¹, Kumaran Santhalingam ², Pallavi Gangwar ², D. Rajasekar ²

Affiliations
1 Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Vels University, Chennai, Tamil Nadu-600117, IN
2 Gensilico Biosolutions, Zamin Pallavarm, Chennai, Tamil Nadu-600043, IN

Abstract

Rheumatoid arthritis is characterised by persistent synovitis, systemic inflammation, and autoantibodies. It is best considered a clinical syndrome spanning several disease subsets. Molecular Docking is a method which predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex.The present study is mainly concentrating the theoretical synthesis of 3-methylquercetin (Isorhamnetin) is an O-methylated flavonol, contain choromone as the basic moiety and found in Tagetes lucida plant used as inhibitors of IL-6 protein which is involved in rheumatoid arthritis, docking was carried out in Argus Lab software. The IL-6 protein structure (PDB ID: 1ALU) was found from protein data bank. The parent compound 3-methylquercetin has shown best ligand pose -7.2703kcal/mol. All the derivatives have shown best ligand pose energy between -6.9293kcal/mol to -10.2862 kcal/mol. Out of the eight 3-methylquercetins derivaties, 1h posses beast ligand pose (-10.2862 kcal/mol).Thus from the theoretical studies imparts that 3, 5, 7-trihydroxy-2-(4-hydroxy-3-methoxyphenyl)-4h-chromen-4-one (3-methylquercetin) derivatives are active towards IL-6 protein and thus used for rheumatoid arthritis.

Keywords

Rheumatoid Arthritis, IL-6 Protein, 3-Methylquercetin, Argus Lab.

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