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Authors
Affiliations
1 College of Resources and Environment, Northwest A and F University, Yangling, 712100, Shaanxi, CN
2 College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji, 721013, Shaanxi, CN
3 Center of Bayreuth Institution for Environment Protection, 7982, Bayreuth, DE
Source
Nature Environment and Pollution Technology, Vol 15, No 1 (2016), Pagination: 97-102
Abstract
Carbendazim (MBC) is one of the most widely used agricultural fungicides worldwide, but this chemical adversely affects animal health and ecosystem function. As such, effective methods for remediating MBCcontaminated soils must be developed. In this study, a novel MBC-degrading bacterium, namely, djl-5B, was isolated from MBC-treated soil. Based on its phenotype features as well as physiological, biochemical, and phylogenetic characteristics, the strain was identified as Pseudomonas sp. The optimal degradation condition for strain djl-5B was determined to be at 30°C and pH 7.5 with an inoculum size of 7%. Inoculation of strain djl-5B to MBC-contaminated soil significantly decreases the degradation half-life of MBC from 14.15 d to 3.06 d in non-sterile soil and from 24.75 d to 3.30 d in sterile soil. Results of high-pressure liquid chromatography-mass spectrometry analysis revealed that the degradation product of strain djl-5B contains 2-aminobenzimidazole, 2-hydroxybenzimidazole and benzimidazole. The newly isolated strain effectively degrades MBC and thus exhibits potential for environmental rehabilitation. This study provides basic information and technical support for future utilization of strain djl-5B as an ecofriendly bioremediation agent.
Keywords
Carbendazim (MBC), MBC Degrading Bacterium, Strain Djl-5b, Degeneration Characteristics.
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