A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Dua, Seema
- Seroprevalence of Transfusion Transmitted (Single and Dual) Infections in Blood Donors of Western U.P. India
Authors
1 Dept. of Pathology, SMS&R, Sharda Hospital Greater Noida U.P, IN
2 Dept. of Microbiology, SMS&R, Sharda Hospital Greater Noida U.P, IN
Source
Indian Journal of Public Health Research & Development, Vol 5, No 3 (2014), Pagination: 307-311Abstract
Background: Blood transfusion service (BTS) is an integral part of health care system which primarily aims at providing adequate and safe blood. Transfusion transmitted infections (TTI) pose constant threat to safety of recipients of blood. Therefore all donated blood should be screened against these infections.
Aims &objectives: This retrospective study was carried out to know seroprevalence of different Transfusion transmitted infections in healthy blood donors of Western U.P.to prevent transmission of diseases.
Material and method: Blood units collected from healthy non remunerated , replacement and voluntary donors within blood bank premises of Sharda hospital, Greater Noida, over a period of 6 years(2007-2012) were screened against NACO recommended TTI- HIV, HBsAg, HCV, Syphilis and Malaria parasite.
Results: Total donations were 9592. Out of which 330 donors were sero reactive and 18 showed co infections (dual infection). Seropositivity for HBsAg was present in 168 donors (1.75%), HCV - 116(1.208%), RPR for syphilis 52 (0.54%) and HIV - 12 cases (0.12%) . Majority of single and co infections were in 21-40 yrs age group .Co infection was present in 18 cases. RPR done for syphilis was a common positive marker in all 18 cases of co infection. Other infections were HBsAg -11 cases (61.1%), HCV - 6 cases (33.3%) and HIV - one case (5.55%).
Conclusion: Blood bank should follow more stringent donor selection criteria and promote voluntary donation. Public awareness regarding mode of spread of TTI and judicious use of blood will reduce the threat of transfusion transmitted infections.
Keywords
Transfusion Transmitted Diseases, Blood Donors, Hepatitis B Surface Antigen, Hepatitis C Virus, Human Immune Deficiency Virus- Simple Somatic Cell Assay to Screen Mammalian Embryo Toxicity Caused by Mineral Oil
Authors
1 Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, IN
Source
Current Science, Vol 117, No 8 (2019), Pagination: 1270-1271Abstract
The success of in vitro fertilization (IVF), mammalian embryo culture and somatic cell nuclear transfer (SCNT) depend on stringent laboratory conditions and quality of chemicals, plastic ware, and glassware used to culture generated embryos. Therefore, all components and conditions need to be regularly monitored to avoid any kind of contamination and toxicity. Mineral oil (MO), a petroleum product, is extensively used to overlay the embryo cultures to maintain pH and osmolality of the culture medium. In human and farm animals, embryos are cultured with MO for 5 to 7 days to develop fertilized embryos to blastocyst stage1. Commercial suppliers claimed that MO is embryo safe by testing it with mouse embryo culture; however, MO quality can varied by a lot number and may deteriorate by improper storage and transport conditions. In addition, MO is an oil product that makes it susceptible to peroxidation and free radical formation2,3. Therefore, embryologist( s) should be more attentive to avoid MO-based embryo contamination and toxicity.References
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