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Kalia, Sanjay
- Rapid Regeneration of Bambusa nutans Munro. from Shoot and Leaf Explants
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Adventitious Buds
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Indian Forester, Vol 135, No 7 (2009), Pagination: 994-1002Abstract
Callus induction was achieved on pre-injured explants. Performance of explants derived from in-vitro multiplying cultures was better than those from mature plants. 2, 4-D proved to be superior in inducing callogenic response than NAA or IBA. Enhancement of callogenic efficiency of in-vitro derived explants was observed when BAP (2.5μM) was supplemented to auxin-enriched medium. Induced callus was multiplied on multiplication medium (5μM 2,4-D+ 1.25μM BAP) for varying periods but optimal multiplication rate for shoot callus (1.69) and leaf callus (1.39) was obtained after subculture cycle of three weeks. Shoot and bud regeneration efficiency of calli induced from leaves and shoot varied as well as their phytohormonal requirements. Buds and shoots were regenerated at higher frequency on shoot calli cultured on multiplication medium supplemented with (5μM BAP+ 1.25μM NAA) whereas for leaf calli it was 7.5μM BAP + 1.25μM NAA. Spontaneous ischolar_main regeneration occurred at frequency of 61% on shoots regenerated on multiplication medium.Keywords
Bambusa nutans, Regeneration, Morphogenic Responses, Organogenic Callus,Adventitious Buds
- Flowering in Exotic Bamboo Dendrocalamus asper in India
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Indian Forester, Vol 127, No 9 (2001), Pagination: 1053-1057Abstract
No abstract- Tissue Culture of 40 Year Old Clump of Bambusa Nutans Munro Culture
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Indian Forester, Vol 138, No 6 (2012), Pagination: 562-566Abstract
Nodal segments taken from 40 yr old clump were surface sterilized using 0.1% (w/v) HgCl2 solution for 15-20 minutes and placed aseptically on media supplemented with 7.5µM BAP for bud break (79.16 ± 7.22) and it was observed within a period of two weeks. Sprouted shoots were excised along with small piece of mother tissue and kept on medium supplemented with 5.0µM BAP for multiplication for 4-5 subculture cycles. Bunch of three shoots exhibited faster shoot multiplication when cultured on multiplication media. During multiplication phase, BAP alone proved to be superior to kinetin. Microshoots exhibited higher ischolar_maining percentages on media supplemented with IBA than other auxins (IAA or NAA). Effective IBA concentration was found to be 10µM on which 72.22% of propagules ischolar_mained. Hardening was effectively carried out on half strength MS media without growth regulators, vitamins and sucrose. Success was achieved in acclimatizing 73.33% ischolar_mained propagules on mixture carrying potting mix of sand:FYM: Soil in l:l:l in poly-tunnels. Acclimatized plantlets were successfully transplanted to pots.Keywords
Bambusa Nutans, Indigenous, Media, Micropropagation- Recent Advances in Understanding the Role of Growth Regulators in Plant Growth and Development in Vitro-I. Conventional Growth Regulators
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Authors
Affiliations
1 Division of Biochemistry, Indian Agricultural Research Institute, Delhi - 110012, IN
2 School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IN
3 Department of Biotechnology, CGO Complex, Lodhi Road, New Delhi – 110053, IN
4 Indian Council of Forestry Research and Education, Dehradun, IN
5 Central Arid Zone Research Institute, Jodhpur – 342003, IN
1 Division of Biochemistry, Indian Agricultural Research Institute, Delhi - 110012, IN
2 School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IN
3 Department of Biotechnology, CGO Complex, Lodhi Road, New Delhi – 110053, IN
4 Indian Council of Forestry Research and Education, Dehradun, IN
5 Central Arid Zone Research Institute, Jodhpur – 342003, IN
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Indian Forester, Vol 142, No 5 (2016), Pagination: 459-470Abstract
Growth regulators, a diverse array of organic compounds, are critical components in determining developmental pathways in plants. They interact at the cellular level to produce physiological and morphological effects. Our understanding about transport, metabolism and mode of action of growth regulators in plants has considerably increased in the recent years. Discovery of the chemicals that interfere with synthesis, transport and action of endogenous growth regulators have further improved our knowledge regarding the role of plant growth regulators (PGRs) in plant's growth and development. A number of PGRs are being used in plant cell, tissue and organ cultures for decades, while many of them have recently been discovered and tested for their effects in vitro. In this review, we attempted to summarize the remarkable progress that has been made over the past decades towards understanding PGRs. The progress is further improving our knowledge of the molecular mechanisms of their action, and beginning to explain how PGRs not only have direct influence on cellular growth, but also control various aspects of plant's growth in vivo as well as in vitro.Keywords
Abscisic Acid, Auxin, Cytokinin, Ethylene, Gibberellins, Plant Growth Regulators.References
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- Recent Advances in Understanding the Role of Growth Regulators in Plant Growth and Development in Vitro - II. Non-Conventional Growth Regulators
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Authors
Affiliations
1 Central Arid Zone Research Institute, Jodhpur – 342003, (Rajasthan), IN
2 School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IN
3 Department of Biotechnology, CGO Complex, Lodhi Road, New Delhi, IN
4 Indian Council of Forestry Research and Education, Dehradun, IN
5 Division of Biochemistry, Indian Agricultural Research Institute, Delhi, IN
1 Central Arid Zone Research Institute, Jodhpur – 342003, (Rajasthan), IN
2 School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IN
3 Department of Biotechnology, CGO Complex, Lodhi Road, New Delhi, IN
4 Indian Council of Forestry Research and Education, Dehradun, IN
5 Division of Biochemistry, Indian Agricultural Research Institute, Delhi, IN
Source
Indian Forester, Vol 142, No 6 (2016), Pagination: 524-535Abstract
A diverse array of growth regulators interact at the cellular level to produce physiological and morphological effects on plant growth, morphology and yield. The five conventional growth regulators viz. auxins, cytokinins, gibberellins, abscisic acid and ethylene are being used in plant cell, tissue and organ cultures for decades, while many of them, like non-purine cytokinins, polyamines, jasmonates, brassinosteroids, oligosaccharides, sterols, phosphoinositosides, salicylic acid and systemins, have recently been discovered and tested for their effects in vitro. However, many have not yet been examined for their effects on in vitro growth and development of plants. In this review, we attempted to summarize the progress that has been made over the past two decades towards understanding the role of non conventional PGRs in plant growth and development.Keywords
Brassinosteroids, Jasmonic Acid, Oligosaccharides, Plant Growth Regulators, Polyamines, Salicylic Acid, Signal Peptides.References
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