Informatics Publishing Limited

Sachin U. Rakesh ¹, Priyanka R. Patil ², Vijay R. Salunkhe ²

Affiliations
1 Govt. College of Pharmacy, Karad- 415 124, Dist. Satara (MS), IN
2 Govt. College of Pharmacy, Karad- 415 124, Dist. Satara. (MS), IN

Abstract

Momordica charantia Linn. is a commonly used herb in Ayurvedic medicine. This review supports all updated information on its phytochemical and pharmacological activities, traditional uses and scientific approach. The plant extracts and its chemical marker or target molecule charantin have been widely used for the treatment of a large number of human ailments. The chemical entities of this plant has been used as an antidiabetic, antihelmintic, antimicrobial, hypotriglyceridemic, hypocholesterolemic, insecticidal, antioxidant, anticancer, antimalarial, larvicidal, antiviral, antiulcerogenic agents. Scientifically proved activities are related with traditional concept. Scientific evidence exists with respect to their major and minor constituents. M. charantia is the most important controversial and effective natural origin that has a tremendous future for research. The novelty and applicability of M. charantia are hidden. Such things should be overcome through modern scientific concept.

Keywords

Momordica charantia, Charantin, Antidiabetic, Photochemistry, Pharmacological Activities.

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Sachin U. Rakesh ¹, V. R. Salunkhe ¹, P. N. Dhabale ¹, K. B. Burade ¹

Affiliations
1 Govt. College of Pharmacy, Vidyanagar, Tal. Karad- 415 124, Dist. Satara, IN

Abstract

A sensitive and reliable high performance thin layer chromatographic method has been developed for quantitation of gallic acid in the dried flowers of Nymphaea stellata willd. The hydroalcoholic extract of N. stellata was chromatographed on silica gel 60 F254 plates with chloroform: ethyl acetate: formic acid, 7.5: 6: 0.5 (v/v/v), as mobile phase. Detection and quantitation were performed by densitrometric scanning at = 292 nm, by using deuterium lamp. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method. The average recovery of gallic acid was found to be 98.33%. The proposed HPTLC method provide a good resolution of gallic acid from other constituents present in hydroalcoholic extract of dried flowers of N. stellata and can be used for quantitation of gallic acid present in extract. The method is rapid, simple and precise.

Keywords

HPTLC, Gallic Acid, Nymphaea stellata Willd.

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Priyanka R. Patil ¹, Sachin U. Rakesh ¹, P. N. Dhabale ¹, K. B. Burade ¹

Affiliations
1 Govt. College of Pharmacy, Karad- 415 124, (Satara), Maharashtra, IN

Abstract

Two simple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of Losartan Potassium and Amlodipine Besylate in tablet dosage form have been developed. First method employs formation and solving of simultaneous equation using 208 nm and 237.5 nm as two analytical wavelengths for both drugs in methanol. The second method is Q value analysis based on measurement of absorptivity at 242.5 nm (as an iso-absorptive point) and 237.5 nm. Losartan Potassium and Amlodipine Besylate at their respective max 208 nm and 237.5 nm and at isoabsorptive point 242.5 nm shows linearity in a concentration range of 2-20 g/mL. Recovery studies range from 99.95% for Losartan Potassium and 99.33% for Amlodipine Besylate in case of simultaneous equation method and 102.93% for Losartan Potassium and 101.02% for Amlodipine Besylate in case of Q - analysis method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

Keywords

Losartn Potassium, Amlodipine Besylate, λMax , Simultaneous Equation Method, Q Analysis.

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