Informatics Publishing Limited

G. Venkata Prasad ¹, S. Sravani ¹, B. Mohammed Ishaq ¹, M. Madhu ¹, Sreenivasulu Munna ¹, C. Gopinath ¹

Affiliations
1 Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, IN

Abstract

To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5μg/ml to 40μg/ml (Method A) in Phosphate Buffer pH 2.0 and 5μg/ml to 50μg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x - 0.021 (Method A) and Y = 0.014x - 0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 μg/mL and 2.85 μg/mL (Method A) and 2.357 μg/mL and 7.857 μg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.

Keywords

Cephalexin, Spectroscopic Method, Phosphate Buffer (Ph 2.0), 0.1 N Hcl

Full Text

     

P. Thamarai Selvi ¹, M. Senthil Kumar ¹, T. Kathiravan ¹, R. Rajesh ¹, J. Megala ¹, S. Sravani ¹

Affiliations
1 Department of Pharmacology, Annai Veilankanni's Pharmacy College, Chennai-15, IN

Abstract

The antistress activity of aqueous extract of Centella asiatica. Linn leaves (Umbelliferae) were studied at doses of 100mg and 200mg/kg against tail suspension test. A dose dependent reduction in immobility time was observed in TST. Imipramine (30mg/kg, i.p) was selected as reference standard. The extract also significantly potentiate the Phenobarbitone induced sleeping time in mice. The findings from the present investigation indicates the extract has significant antistress as shown by its effects on different experimentally induced animal models.

Keywords

Centella asiatica, Antistress, Tail Suspension Test, Phenobarbitone Induced Sleeping Time.

Full Text

     

K. Vijaya Sri ¹, S. Sravani ¹, M. Shiva Kumar ¹

Affiliations
1 Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Secunderbad, Telangana, IN

Abstract

The UV-spectroscopic method were developed and validated for the estimation of Lurasidone HCl as per ICH guidelines. A simple, fast, accurate and precise UV-spectroscopy method was developed by using methanol: water (70:30v/v) and acetonitrile: water (50:50v/v) was used as the solvent in both methods. The λ_max of Lurasidone was found to be 230 nm and it was proved linear in the concentration range of 2.5-15 μg/ml with a correlation coefficient value of 0.999 for both the methods. The accuracy studies of UV-spectroscopy method was performed at three different levels, i.e., 50%, 100%, and 150% and recovery was found to be in the range of 99.2 to 102.4% respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.61-0.69 for both UV Method. The % RSD is <2% which indicates the accuracy and precision of the method.

Keywords

Lurasidone, Validation, Estimation, UV Spectrophotometry, Methanol: Water and Acetonitrile: Water.

Full Text