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K. Narasimha Rao ¹, B. Vijaypal Reddy ¹, S. Girisham ¹, S. M. Reddy ¹

Affiliations
1 Microbial Biotechnology Research Laboratory, Dept. of Microbiology, Kakatiya University, Warangal – 506009, Andhra Pradesh, IN

Abstract

Influence of different media, pH and different nutrients on growth and fumonisins (B1) production by Fusarium moniliforme was investigated. The toxin production was analyzed by Thin Layer Chromatography method. Among different media studied Nash and Synders medium, pH of 7.5 and malt extract at concentration of 0.5% was found to be optimum for maximum production of fumonisins. The study suggests the significance of these factors hence it is concluded that factors should be optimized for maximum production of fumonisins.

Keywords

Fumonisins (B₁), Fusarium moniliforme, Mycotoxin

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Ramchander Merugu ¹, M. S. K. Prasad ¹, D. Vasavi ², S. Girisham ², S. M. Reddy ²

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal-506 009, A.P., IN
2 Department of Microbiology, Kakatiya University, Warangal-506 009, A.P., IN

Abstract

Four anoxygenic phototrophic bacteria, Rhodobacterium (Rb.) capsulatus, Rhodopseudomonas (Rps.) acidophila, Rps. rutila and Rhodospirillum (Rsp.) rubrum, isolated from wastewaters, were tested for their ability to solubilize tricalcium and dicalcium phosphate under different conditions. Rps. rutila was superior to other bacteria under investigation in solubilisation of tricalcium phosphate. Rsp. rubrum was poor in solubilization of tricalcium phosphate. Rsp. rubrum and Rb. capsulatus could solubilise dicalcium phosphate with almost same efficiency while Rps. acidophila was more efficient in solubilization of dicalcium phosphate. Thus, these bacteria can be exploited in solubilization of phosphate.

Keywords

Rb. capsulatus, Rps. acidophila, Rps. rutila, Rsp. rubrum, Phosphate Solubilization, Dicalcium Phosphate, Tricalcium Phosphate.

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Ramchander Merugu ¹, M. S. K. Prasad ¹, S. Girisham ², S. M. Reddy ²

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal-506 009, A.P., IN
2 Department of Microbiology, Kakatiya University, Warangal-506 009, A.P., IN

Abstract

The tolerance of some pesticides by two anoxygenic phototrophic bacteria, isolated from leather industry effluents, was investigated. Rhodobacterium capsulatus was found to be more resistant than Rhodopseudomonas acidophila to the pesticides tried in the present investigation. The pattern of inhibition of growth for both the organisms show that chlorpyrifos was most potent followed by methyl parathion, endosulfan, rogor and kitazin in a descending order.

Keywords

Pesticides, Biofertilizers, Rb. capsulatus, Rps. Acidophila, Leather Industry Effluent.

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Ramchander Merugu ¹, M. S. K. Prasad ¹, S. Girisham ², S. M. Reddy ²

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal-506 009, A.P., IN
2 Department of Microbiology, Kakatiya University, Warangal-506 009, A.P., IN

Abstract

The presence of photosynthetic bacteria along with the heterotrophic bacteria has been reported in various aquatic environments like Indian tropical waters, salt marshes, industrial effluents, seawater, sewage water and hot water springs. Two anoxygenic phototrophic bacteria Rb. capsulatus and Rps. acidophila were isolated from leather industrial effluents and influence of some heavy metals on the growth of these two bacteria was studied. Iron and cadmium requirement for the growth of Rb. capsulatus and Rps. acidophila was very low, while selenium and cerium failed to influence the growth of both the bacteria under investigation. Rps. acidophila was highly sensitive to and was totally inhibited by mercuric chloride at a very low concentration, whereas Rb. capsulatus exhibited considerable resistance. Biological significance of above observations in the light of existing literature is discussed.

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