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S. Chandra , N. S. K. Harsh

Abstract

No abstract

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B. Poornasri Devi ¹, A. Vimala ¹, Isha Sai ¹, S. Chandra ²

Affiliations
1 Dept. of Biosciences, Sri Sathya Sai University, Anantapur Campus, Anantapur-5150 001, A.P, IN
2 P.G. Dept. of Botany, Queen Mary's College, Chennai 600004,T.N, IN

Abstract

The present study aimed to elucidate the effect of cyanobacterial elicitor derived from Anabaena sp. and Nostoc carneum on neem cell suspensions evolved from superior callus lines of Azadirachta indica A. Juss elite KN-1 accession with 12.88 mg/gm azadirachtin in its seed. Neem cell suspension cultures maintained in Murashige and Skoog liquid medium were treated with 265 cells/ml and 530 cells/ml homogenate of the elicitor on the third day. Growth of neem callus in terms of biomass revealed 5 fold enhancements when treated with 530 cells/ml elicitor on tenth day in callus line-1. In callus line-2 the log phase extended till tenth day recording a 6 fold increase with 265 cells/ml elicitor concentration. The type of callus line and concentration of the elicitor influence the biomass growth of neem callus in suspension culture. Enhanced azadirachtin production was achieved by algal elicitor treatment (as confirmed by HPLC analysis) and this study makes a step forward towards industrial manufacturing of the neem alkaloid.

Keywords

Anabaena sp., Nostoc Carneum, Neem, Azadirachtin, Elicitor, Suspension Culture

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A. Vanitha ¹, A. Premkumar , S. Chandra ¹, R. Tamil Selvi ¹

Affiliations
1 PG & Research Department of Botany, Queen Mary’s College, Chennai-600 004, TN, IN

Abstract

Bryocladia thwaitesii (Harvey ex J. Agardh) Detoni, is a commercially important red algae finding wide use in pharmaceutical, cosmetics, nutraceutical industries. However there is no work was made on the DNA isolation which is the key step for further improvement of its yield. Here, for the first time we report the DNA isolation suitable for molecular biological studies and the quality and quantity of the DNA is reported in comparison with the green alga (Ulva covelongenesis V. Krishnamoorthy and H.Joshi). Optimization of DNA isolation was done in these algae by adopting different methods. In the optimization process, different DNA isolation methods were followed. A better result in terms of purity and yield was obtained in method suggested by Wattier et al. (2000) modification by Dellaporta et al. (1983). Quantitative analysis made using Nano vue UV spectrophotometer sets as a pioneering effort in the B. thwaitesii. The DNA isolated by this protocol from two seaweeds, both the purity of DNA as well as concentration were very high i.e., 1.772 and 594.5 mg/μl respectively in B. thwaitesii. In U. covelongenesis the purity of DNA was 1.763 and concentration of DNA 569.5 mg/μl, which was fairly high in other protocols studied, the purity of DNA ranged from 1.444 to 1.640 and concentration of DNA ranged from 71 to 463 ngm/μl. All the experiments were performed by keeping the green alga U. covelongenesis as standard for comparison. The DNA isolation protocol indentified in the present investigation in the seaweed B. thwaitesii would become well suited for PCR amplification and genomic library construction.

Keywords

Bryocladia thwaitesii, Ulva covelongenesis, DNA Isolation, Nano Vue UV Spectrophotometer

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S. Sujanya ¹, S. Chandra ¹

Affiliations
1 PG Dept, Queen Marys College, Chennai-600004, Tamil Nadu, IN

Abstract

Field trials were conducted during the years 2009-2010, at a private farm in Puttaparthi, Anantapur District, India, to study the effect of biological agents as effective seed treatment substitutes over commonly used chemicals on ground nut plants,Arachis hypogea. The biological agents (BA) tested for this purpose involved, BGA,Azotobacter and Azospirillum. Seeds were treated prior to sowing with each of these 3 agents both individually and in combination. Commonly used chemical for seed treatment, was used as the control. The treated seeds were sown on the soil combination, 3:1 FYM: NPK. BGA proved to be the best among the 3 biological agents. The effect of Azotobacter was marginal when used alone. However, the same was augmented in the presence of BGA. Azospirillum seemed to have a negative effect on growth and yield, when applied alone and in combination with BGA.

Keywords

Biofertilizer, Arachis Hypogea, Yield FYM, NPK, BA, BGA

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A. Purushottam ¹, B. V. S. R. Murty ¹, S. Chandra ¹, N. Subba Rao ², A. Syamala Rao ², P. C. Basu ², N. K. Sood ²

Affiliations
1 Chemical Division, Geological Survey of India, 4-3-542, Bogulkunta, Hyderabad 500001, IN
2 Division of Regional Integrated Survey, Geological Survey of India, 4-3-542, Bogulkunta, Hyderabad 500001, IN

Abstract

Sequential extraction techniques for determination of trace metals associated with different phases in soils/stream sediments are being increasingly used for geochemical exploration in recent years. The existing techniques do not provide for the separate determination of Copper, Lead or Zinc present in the secondary mineral form, although in many soils and stream sediments, these metals exist in such phases. Based on laboratory studies, the sequential extraction technique suggested by Chao and Theobald (Jr) has been modified to include an additional step to provide for determination of Copper, Lead and Zinc present in their commonly occurring secondary mineral forms. Making use of the modified scheme, stream sediment samples from Ahobilam area in Cuddapah Proterozoic basin have been analysed. Metal contents of samples in the primary and secondary mineral phases were found to be good indicators for Pb and Zn mineralization in zones 2 and 3. Subsequent field check revealed Ph-Zn mineralization in t he above two zones.

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S. Chandra ¹

Affiliations
1 Botanical Survey of India, Howrah, IN

Abstract

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S. Chandra ¹, K. L. Maity ¹

Affiliations
1 Botanical Survey of India, Howrah, IN

Abstract

No Abstract.

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S. Chandra ¹, S. Atal ¹, N. S. Krishnamurthy ¹, k. Subrahmanyam ¹, R. Rangarajan ¹, D. V. Reddy ¹, P. Nagbhushanam ¹, J. V. S. Murthy ¹, S. Ahmed ¹, V. P. Dimri ¹

Affiliations
1 National Geophysical Research Institute, Hyderabad - 500 007, IN

Abstract

No Abstract.

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P. Kuppusamy ¹, B. Kalaavathi ², S. Chandra ³

Affiliations
1 Department of Computer Science and Engineering, Gnanamani College of Technology, IN
2 Department of Computer Science and Engineering, K.S.R Institute for Engineering and Technology, IN
3 Department of Computer Science, Selvamm Arts and Science College, IN

Abstract

A cooperative caching approach improves data accessibility and reduces query latency in Mobile Ad hoc Network (MANET). Maintaining the cache is challenging issue in large MANET due to mobility, cache size and power. The previous research works on caching primarily have dealt with LRU, LFU and LRU-MIN cache replacement algorithms that offered low query latency and greater data accessibility in sparse MANET. This paper proposes Memetic Algorithm (MA) to locate the better replaceable data based on neighbours interest and fitness value of cached data to store the newly arrived data. This work also elects ideal CH using Meta heuristic search Ant Colony Optimization algorithm. The simulation results shown that proposed algorithm reduces the latency, control overhead and increases the packet delivery rate than existing approach by increasing nodes and speed respectively.

Keywords

Data Replacement, Clustering, Cooperative Caching, Optimization.

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S. K. Yadav ¹, S. K. S. Rajput ², M. Tripathi ³, R. Yadav ⁴, S. Chandra ⁵, R. K. Singh ¹

Affiliations
1 Krishi Vigyan Kendra, Azamgarh (U.P.), IN
2 Krishi Vigyan Kendra, Sonbhadra (U.P.), IN
3 Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad (U.P.), IN
4 Krishi Vigyan Kendra, Masoudha, Faizabad (U.P.), IN
5 Department of Genetics and Plant Breeding, Narendra Dev University of Agricultural and Technology, Kuamrganj, Faizabad (U.P.), IN

Abstract

Frontline demonstration (FLD) was conducted at Krishi Vigyan Kendra, Azamgarh, Uttar Pradesh on chickpea (pulse) using seed plus phosphate solublizing bacteria (PSB), Rhizobium plus Trichoderma plus insecticide (indoxacarb) in combination, at farmers fields for two consecutive years (2013-14 and 2014-15). The highest grain yield (14.10 q/ha) was observed in varietyPG-186 in year 2014-2015. It was 46.9 per cent higher yield over the farmers practice (9.60 q/ha). The study indicates FLD enhances the productivity of chickpea over conventional farmer field methods in each progressive year. The highest grain yield was observed in 2014-15 which was close to yield obtained in 2013-14. The lowest yield was recorded in the year 2013-14 under demonstration when compared to traditional chick pea production method which resulted more than succeeding year (in farmers practice). The increasing trend in the per cent increase of yield was found due to variation in agro-climatic factors under rainfed condition. The FLD produces a significant positive result and provide an opportunity to demonstrate the productivity potential and profitability of the latest technology under existing farming conditions.

Keywords

Chickpea, PSB, FLD, Rhizobium, Trichoderma.

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K. P. Singh ¹, G. C. Dewangan ², S. Chandra ¹, S. Bhattacharayya ¹, V. Chitnis ¹, G. C. Stewart ³, N. J. Westergaard ⁴

Affiliations
1 Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, IN
2 Inter-University Centre for Astronomy and Astrophysics, Pune 411 007, IN
3 Department of Physics and Astronomy, University of Leicester, Leicester, GB
4 Technical University of Denmark, National Space Institute, DK

Abstract

The Soft X-ray focusing Telescope (SXT) is a moderate-resolution X-ray imaging spectrometer supplementing the ultraviolet and hard X-ray payloads for broadband studies of cosmic sources with AstroSat. Well suited for observing bright X-ray sources, SXT observations of nearby active galactic nuclei (AGN), binary star systems with compact companions, active stars, etc. are producing long soft X-ray light curves and high-quality spectra. The strong X-ray variability and multiple spectral components exhibited by SXT observations of nearby Seyfert 1 galaxies show excellent promise to probe accretion disks and central engines in AGN through multi-band variability and spectroscopy.

Keywords

Active Galactic Nuclei, Stars, Supernova Remnants, X-Ray Astronomy.

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